The Product Information Sheet for H9268 advises, "Stock solutions in 0.9% NaCl at 1 mg/mL remain active at least one year stored at 2–8°C." There is no data indicating the stability of a solution in DPBS stored at -80°C.
Fortfahren mit
H9268
Hexadimethrinbromid
≥94% (titration)
Synonym(e):
1,5-Dimethyl-1,5-diazaundecamethylen-polymethobromid, Polybren
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Unterstützung erhaltenQuality Segment
assay
≥94% (titration)
storage temp.
2-8°C
SMILES string
BrCCCBr.N(CCCCCCN(C)C)(C)C
InChI
1S/C10H24N2.C3H6Br2/c1-11(2)9-7-5-6-8-10-12(3)4;4-2-1-3-5/h5-10H2,1-4H3;1-3H2
InChI key
KZKAYEGOIJEWQB-UHFFFAOYSA-N
General description
Hexadimethrinbromid ist eine quarternäre Ammoniumverbindung, die als Heparin-Antagonist wirkt. Es ist zudem an der Transformation von DNA mit niedrigem Molekulargewicht beteiligt.
Application
Hexadimethrinbromid kann zur Transfektion von Säugetierzellen mit DNA verwendet werden. Es kann verwendet werden, um die Effizienz einer Lipofektion zu erhöhen.
Hexadimethrinbromid wurde für die Infektion von Zellen mit Lentivirus verwendet. Es wurde für die Infektion von Zellen mit retroviralem Überstand verwendet.
Hexadimethrinbromid wurde für die Infektion von Zellen mit Lentivirus verwendet. Es wurde für die Infektion von Zellen mit retroviralem Überstand verwendet.
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| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
| assay ≥94% (titration) | assay - | assay ≥95% | assay - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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After reconstituting H9268 in DPBS, aliquoting and storing at -80C, how long is the solution good for?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHilfreich?
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Is it possible for hexadimethrine bromide to breakdown and lose its effectiveness after 3 years when stored in its powdered form at 4 degree celsius? We bought this product around 3 years and now it doesn't work as well as before when doing transduction.
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This product is assigned a recommended retest period of 2 years from the date of Quality release. While quite stable, the compound may slowly degrade over time. The suitability of this item beyond the lot specific retest date would have to be determined by the end user.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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what is the concentration of 5G polybrene? Does G stands for gram?
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This product is in powder form and not a prepared solution. The minimum purity of this material is 94%. The 'G' represents grams.
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What is the preferred solvent for Hexadimethrine bromide? I see water or saline above, but does either effect solubility or stability?
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The choice of solvent typically depends on the specific application. For in vitro or in vivo studies, saline is more commonly used. The material is soluble in water at up to 10%. Solutions are quite stable and maintain activity after autoclaving. Stock solutions prepared at 1 mg/mL in saline are stable for up to 1 year when stored at 2–8 °C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/253/063/h9268bul.pdfHilfreich?
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How long is the Hexadimethrine Bromide powder stable? I cannot find its expiry date.
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This product in powdered format is stable for up to a recommended retest period of two years as listed on the Certificate of Analysis. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.
When in solution, this product will remain active for at least one year if stored at 2–8 °C. For more information please view the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/253/063/h9268bul.pdf
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Is Product H9268, Hexadimethrine bromide, the same thing as Polybrene?
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Yes, Polybrene (registered trade-mark of Abbott) and 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide, are Synonyms.
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How do I sterilize Product H9268, Hexadimethrine bromide?
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Hexadimethrine bromide can be filtered sterilized or autoclaved.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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