SRP0263

PDE4B1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Synonym(e): DPDE4, PDE4B, Phosphodiesterase 4B, cAMP-specific, Phosphodiesterase 4B1
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected insect cells
• Assay: ≥70% (SDS-PAGE)
• Form: aqueous solution
• Spezifische Aktivität: ≥10000 units/μg
• Mol-Gew.: 109 kDa
• Verpackung: pkg of 10 μg
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_001037341
• UniProt-Hinterlegungsnummer: Q07343
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... PDE4B(24626)

Beschreibung

Allgemeine Beschreibung

Human PDE4B1 (GenBank Accession No. NM_001037341), full length with N-terminal GST tag, MW = 109 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Einheitendefinition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 30°C

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable