SRP0135

G9a human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Synonym(e): EHMT2, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, KMT1C, Lysine N-methyltransferase 1C
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥70% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 74.6 kDa
• Verpackung: pkg of 50 μg
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_006709
• UniProt-Hinterlegungsnummer: Q96KQ7
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... EHMT2(10919)

Beschreibung

Allgemeine Beschreibung

Human G9a (euchromatic histone-lysine N-methyltransferase 2) with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system. G9a is a member of SET (Su(var)3-9, enhancer-of-zeste and trithorax) domain containing, Su(var)3-9 family of proteins. This domain is evolutionary conserved.[1]

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem./physiol. Wirkung

The encoded protein G9a (euchromatic histone-lysine N-methyltransferase 2) is a methyltransferase that methylates lysine residues of histone H3.[2] Overexpression of G9a is observed in cancer. It is known to participate in cancer cell migration, invasion and metastasis.[3] G9a is an epigenetic regulator and can inhibit the action of tumor suppressor genes, thereby, contributes to carcinogenesis. Thus, G9a is considered to be an oncogenic and metastatic factor.[4] It also affects DNA damage repair mechanism, which might make tumor cells resistant to radiation and chemotherapy.[5] G9a serves as a target for cancer therapy including breast cancer.[4]

Einheitendefinition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable