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SHC001V

Sigma-Aldrich

MISSION® pLKO.1-puro Empty Vector Control Transduction Particles

Contains no shRNA insert

Synonym(e):

MISSION®, MISSION® Control Transduction Particles

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About This Item

UNSPSC-Code:
41106609
NACRES:
NA.51

Qualitätsniveau

100

Produktlinie

MISSION®

Konzentration

≥1x106 VP/ml (via p24 assay)

Methode(n)

capture ELISA: 106 TU/mL using p24

Versandbedingung

dry ice

Lagertemp.

−70°C

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Allgemeine Beschreibung

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Anwendung

To see more application data, protocols, vector maps visit sigma.com/shrna.

Rechtliche Hinweise

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Efficacy of adjuvant chemotherapy for non-small cell lung cancer assessed by metastatic potential associated with ACTN4.
Miura N
Oncotarget (2016)
Deepak Parashar et al.
Cancer research, 80(24), 5554-5568 (2020-10-23)
Peritoneal spread is the primary mechanism of metastasis of ovarian cancer, and survival of ovarian cancer cells in the peritoneal cavity as nonadherent spheroids and their adherence to the mesothelium of distant organs lead to cancer progression, metastasis, and mortality.
Soft drug-resistant ovarian cancer cells invade via two distinct mechanisms utilizing myosin IIB
Aastha Kapoor
Nature, 099655-099655 (2017)
Chemoresistance is associated with increased cytoprotective autophagy and diminished apoptosis in bladder cancer cells treated with the BH3 mimetic (-)-Gossypol (AT-101).
Mani J
BMC Cancer, 15, 224-224 (2015)
Xiaobo Luo et al.
The Journal of clinical investigation, 130(4), 1635-1652 (2019-12-25)
The incidence of human papillomavirus-positive (HPV+) head and neck squamous cell carcinoma (HNSCC) has surpassed that of cervical cancer and is projected to increase rapidly until 2060. The coevolution of HPV with transforming epithelial cells leads to the shutdown of
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Artikel

Examination of Lentiviral Library Efficiency

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Protokolle

Protokoll zur lentiviralen Transduktion

Detailliertes Verfahren zur Durchführung einer lentiviralen Transduktion von MISSION shRNA lentiviralen Partikeln, um eine stabile, langfristige Stilllegung und Phänotypveränderung zu erreichen.

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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