SBR00036

Rhodamine B labeled Polymyxin B Ready Made Solution

For fluorescent microbial imaging, 1.1 mg/mL in water

• Synonym(e): SRB-PMB
• UNSPSC-Code: 12352211
• NACRES: NA.47

Eigenschaften

• Qualitätsniveau: 100
• Konzentration: 1.1 mg/mL in water
• Lipid-Typ: saturated FAs
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Rhodamine B labeled polymyxin B (SRB-PMB) is a fluorescent derivative of Polymyxin B. Rhodamine B polymyxin B is an additional fluorescent derivative of Polymyxin B, along with Dansyl labeled polymyxin B (Product # SBR00029). Both products′ mode of action is in accordance with Polymyxin B activity. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore.

Anwendung

Fluorescent antibiotics can be used for many applications[1] including:

• Antimicrobial resistance research.
• Bacterial visualization and imaging.
• Parent antibiotic mode of action research and new antibiotic discovery.
• Toxicity studies.
• Research of bacterial infections and tracking its uptake in vivo.

Additional possible applications for Rhodamine B labeled Polymyxin B:

• Evaluate LPS binding by FRET (fluorescence resonance energy transfer) studies to form a specific complex between fluorescein (FITC)-tagged LPS and Polymyxin B, indicating direct binding of the antibiotic to the LPS
• Can be applied as a fluorescent probe to study polymyxin mode of action and its pharmacokinetics
• to assess the mitochondrial function of polymyxin B in a kidney cell line (LLC-PK1), suggesting that it changes the mitochondrial membrane polarization.[2]

Hinweis zur Analyse

• Rhodamine B labeled polymyxin B Ready Made Solution is light sensitive.
• It is recommended to avoid freeze-thaw cycles of Rhodamine B labeled polymyxin B Ready-Made Solution
• Rhodamine B labeled polymyxin B Ready Made Solution (1.1mg/mL) can be diluted 1:100-200 in PBSX1 (Product# D8537) to achieve 0.55-1.1μg/mL final concentration for staining. The above concentration of Rhodamine B labeled polymyxin B was used for Escherichia coli staining (see image)
• Fluorescence Microscopy application: Rhodamine B labeled polymyxin B Ready Made Solution excitation (Ex) wavelength is 550-560nm resulting in emission (Em) range of 580-610nm (λ_max=590-600nm)

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable