indirect ELISA: 3-6 μg/mL using Human IgG1 myeloma protein for coating.
Isotyp
IgG2a
Versandbedingung
dry ice
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
Allgemeine Beschreibung
Human IgGs consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies. Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. IgG1 and IgG3 are the only subclasses capable of adherence to mononuclear phagocytes and are recognized readily by the Fc receptors on various reticulo-endothelial cells while IgG2 and IgG4 are far less efficient. The amount of the different IgG subclasses present in the bloodstream varies with age. For example, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels raise more slowly, reaching adult levels at about 10 years of age. Serum IgG subclass deficiencies have been recorded for different patient groups. For example, a disproportionate elevation of IgG1 has been found in the cerebral spinal fluid of patients with multiple sclerosis. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and thus may assist in the diagnosis of various disorders.
Immunogen
The Fc fragment of a human IgG1 myeloma protein.
Anwendung
Indirect ELISA: a working concentration of 3-6 μg/mL is recommended using 10 μg/mL Human IgG1 myeloma protein for coating.
Physikalische Form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.
Sonstige Hinweise
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
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