SAB4200203

Anti-RAVER1 antibody, Mouse monoclonal

clone RAV1, purified from hybridoma cell culture

• Synonym(e): Anti-Ribonucleoprotein PTB-binding 1
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: unconjugated
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: RAV1, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~80 kDa
• Speziesreaktivität: human, monkey
• Konzentration: ~1.0 mg/mL
• Methode(n): immunoprecipitation (IP): suitable; western blot (chemiluminescent): 1.0-2.0 μg/mL using HeLa or HepG2 cells extract
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: Q8IY67
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... RAVER1(125950)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-RAVER1 (mouse IgG1 isotype) is derived from the hybridoma RAV1 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from mouse immunized with a peptide corresponding to a fragment of human RAVER1. Raver1, also known as ribonucleoprotein PTB-binding 1, is a widely expressed multidomain protein, identified in two-hybrid screens by its ability to interact and colocalize with the cytoskeletal proteins -actinin and vinculin. The protein is composed of three RNA recognition motifs (RRM) and of nuclear localization and nuclear export signals, allowing it to shuttle between the nucleus and the cytoplasm. Raver1 also colocalizes with PTB/hnRNPI.

Anwendung

Monoclonal Anti-RAVER1 antibody produced in mouse has been used in immunoblotting.

Biochem./physiol. Wirkung

RAVER1 (ribonucleoprotein, PTB binding 1) is involved in RNA splicing of microfilament proteins. In skeletal muscle, a translocation of Raver1 from the nucleus to the cytoplasm is correlated with the differentiation of specific microfilament attachment sites. Based on an analysis of Vinculin-Raver1 crystal structure it was suggested that vinculin recruits raver1 and its mRNAs cargo to focal adhesions, promoting localization of the synthesis of adhesion complexes by the translational machinery.

Physikalische Form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable