S5933

Anti-STAT3 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL-Nummer: MFCD01092492
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: IgG fraction of antiserum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 92 kDa
• Speziesreaktivität: human, rat, mouse
• Methode(n): immunocytochemistry: 10 μg/mL using A431 cells; immunoprecipitation (IP): 4 μg using STAT3 from 0.5–1 mg of an EGF stimulated human A431 cell lysate; western blot (chemiluminescent): 0.5-2 μg/mL using lysates from EGF stimulated human A431, mouse WEHI and rat L6 cells
• UniProt-Hinterlegungsnummer: P40763
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... STAT3(6774) ; mouse ... Stat3(20848) ; rat ... Stat3(25125)

Beschreibung

Allgemeine Beschreibung

Signal transducer and activator of transcription 3 (STAT3) protein is a member of the STAT family of proteins. It consists of a DNA binding domain, a Src homology-2 (SH-2) domain, an N-terminal coiled-coiled domain, and a C-terminal transactivation domain. The STAT3 gene is located on the human chromosome at 17q21.2.

Spezifität

The antibody is specific for STAT3. It may detect an additional 66 kDa band using high concentrations or long exposures. The sequence recognized by the antibody (688-722) is identical in mouse and rat STAT3. The first 28 amino acids are identical to mouse STAT3B.

Immunogen

GST fusion protein corresponding to amino acids 688-722 of human STAT3, expressed in bacteria.

Anwendung

Anti-STAT3 antibody produced in rabbit has been used in western blotting.

Biochem./physiol. Wirkung

Signal transducer and activator of transcription 3 (STAT3) signaling participates in cancer inflammation. This protein interacts with nuclear factor-κB (NF-κB). STAT3 regulates the function of several genes that take part in proliferation, apoptosis, metastasis, angiogenesis, immune response, and cell differentiation. Overexpression of the STAT3 gene is associated with lymphomas, prostate carcinoma, breast cancer, hepatocellular carcinoma, pancreatic adenocarcinoma, cholangiocarcinoma, and ovarian carcinoma.

Physikalische Form

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl and 0.05% sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable