Direkt zum Inhalt
Merck

RAB0188

Sigma-Aldrich

Human FGF-7 ELISA Kit

for serum, plasma, cell culture supernatant and urine

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise

Größe auswählen

1 KIT
528,00 €

528,00 €


Check Cart for Availability


Größe auswählen

Ansicht ändern
1 KIT
528,00 €

About This Item

UNSPSC-Code:
41116158
NACRES:
NA.32

528,00 €


Check Cart for Availability

Speziesreaktivität

human

Verpackung

kit of 96 wells (12 strips x 8 wells)

Methode(n)

ELISA: suitable
capture ELISA: suitable

Aufnahme

sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.5 pg/mL
standard curve range: 0.55-400 pg/mL

Nachweisverfahren

colorimetric

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

human ... FGF7(2252)

Allgemeine Beschreibung

The Human FGF-7 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF-7 in serum, plasma, cell culture supernatants and urine.
The gene FGF7 (fibroblast growth factor 7) is mapped to human chromosome 15q21.1.[1] The encoded protein belongs to the fibroblast growth factor family and is secreted by mesenchymal cells.[2][3]

Immunogen

Recombinant Human FGF7

Anwendung

Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem./physiol. Wirkung

FGF7 (fibroblast growth factor 7) is involved in the development of skin and sweat glands, wound healing and cutaneous homeostasis.[4] It controls keratinocyte differentiation and viability by modulating autophagy.[2] FGF7 also participates in embryonic development, tumorigenesis, cell proliferation, migration and differentiation.[3] It is linked with several disorders like nonsyndromic cleft lip and palate, thyroid volume, goiter risk and chronic obstructive pulmonary disease. FGF7 plays an important role in the morphology of developing lungs. Absence of the FGF7 activity decreases airway branching during development. During injury, it is involved in airway repair.[5]

Sonstige Hinweise

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDB

  • RABSTOP3ELISA Stop Solution (Item I)SDB

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDB

  • RABWASH420X Wash Buffer (Item B)SDB

Piktogramme

Corrosion

Signalwort

Warning

H-Sätze

P-Sätze

Gefahreneinstufungen

Met. Corr. 1

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials


Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Association between fibroblast growth factor 7 and the risk of chronic obstructive pulmonary disease
Xu SC, et al.
Acta Pharmacologica Sinica, 33(8), 998-1003 (2012)
FGF7 Over Expression is an Independent Prognosticator in Patients with Urothelial Carcinoma of the Upper Urinary Tract and Bladder
Fan EW, et al.
The Journal of Urology, 194(1), 223-229 (2015)
Recombinant keratinocyte growth factor 1 in tobacco potentially promotes wound healing in diabetic rats
Feng ZG, et al.
BioMed Research International (2014)
Oncogenic mutations of ALK kinase in neuroblastoma
Chen Y, et al.
Nature, 455(7215), 971-974 (2008)
Role of Keratinocyte Growth Factor in the Differentiation of Sweat Gland-Like Cells From Human Umbilical Cord-Derived Mesenchymal Stem Cells.
Xu Y
Stem Cells Translational Medicine, 5(1), 106-116 (2016)

Fragen

1-3 von 3 Fragen  
  1. What is the specificity of the Human FGF7 ELISA antibodies (RAB0188)?

    1 Antwort
    1. The capture antibody utilized in this kit is a mouse monoclonal antibody with an immunogen of aa32-194 of human FGF-7 (accession # P21781). Meanwhile, the detection antibody is a goat polyclonal antibody with the immunogen of aa32-194 of human FGF-7 (accession # Q6FGV5).

      Hilfreich?

  2. Will the Human FGF-7 ELISA kit (BAB0188) detect mouse FGF7?

    1 Antwort
    1. The Human FGF-7 ELISA kit has not been tested for cross-reactivity to mouse, so we cannot confirm if it can detect mouse FGF-7. However, we do offer a Mouse FGF-7 ELISA with the catalog number RAB1123.

      Hilfreich?

  3. Is there a detailed protocol for this (the general sandwich protocol is not specific enough)? E.g. the kit FGF-7 concentrate for the standard curve - no concentration is written on the vial. And there is no information on how to make serial dilutions.

    1 Antwort
    1. Using the lot number on the product you purchased, you can access the lot-specific CofA to view the procedure and concentration. Example CofA using lot number 0922J0245: https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0188_0922J0245.pdf

      Hilfreich?

Bewertungen

Kein Beurteilungswert

Aktive Filter

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.