R9894

Ras human

Wild type, recombinant, expressed in E. coli, buffered aqueous glycerol solution

• Synonym(e): H-Ras, rH-RAS, WT
• MDL-Nummer: MFCD00241858
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in E. coli
• Assay: ≥80% (SDS-PAGE)
• Form: buffered aqueous glycerol solution
• Mol-Gew.: 28 kDa
• UniProt-Hinterlegungsnummer: Q0VAM2
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... RASGEF1B(153020)

Beschreibung

Allgemeine Beschreibung

Ras proto-oncogene is a guanine-nucleotide binding protein[1] that couples tyrosine kinase receptor signals to mitogen activated protein (MAP) kinase cascade. It has an important role in cell growth.[2] The Ras gene is localized on human chromosome 11p15.5.[3] Ras is a substrate for farnesyltransferase (FTase) and when farnesylated, it is anchored to the membrane.[2]

Anwendung

Human Ras has been used as a negative control in tubulin overlay assay.[4]

Biochem./physiol. Wirkung

Ras proteins, p21, are guanyl nucleotide binding proteins that play an important role in cellular signal transduction.[5] Ras proteins are regulatory proteins that bind GTP in their active state. A Ras intrinsic GTPase activity hydrolyzes guanosine-5′-triphosphate (GTP) to guanosine diphosphate (GDP)[6] thus inactivating Ras, while activation of Ras requires exchange of the bound GDP with GTP. Inhibition of Ras activity by stimulation of its GTPase activity is enhanced by GTPase activating proteins.[7] Membrane localization of Ras is crucial for its cellular activity. Inhibition of Ras farnesylation is a target for anti-cancer drug development, since abnormally active Ras is present in more than 50% of human cancers.

Verpackung

Package size based on protein content

Physikalische Form

Solution in 50% glycerol containing 50 mM Tris (pH 8.0), 5 mM MgCl₂, 45 mM NaCl, 1 mM DTT

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable