Alle Fotos(1)
Wichtige Dokumente
R7260
Xba I from Xanthomonas badrii
Restriction Enzyme
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
Empfohlene Produkte
![[4-(acetylamino)phenyl]acetic acid AldrichCPR](/deepweb/assets/sigmaaldrich/product/structures/998/245/c05afb37-e406-430e-b87e-95b0335140f4/640/c05afb37-e406-430e-b87e-95b0335140f4.png)
Qualität
for molecular biology
Form
buffered aqueous glycerol solution
Konzentration
10,000 units/mL
Versandbedingung
wet ice
Lagertemp.
−20°C
Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich
Spezifität
Recognition sequence: 5′-T/CTAGA-3′
Cutting results: A 2-10-fold Xba I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting
Heat inactivation: Up to 15 units of enzyme inactivated at 65 °C for 15 minutes.
Cutting results: A 2-10-fold Xba I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting
Heat inactivation: Up to 15 units of enzyme inactivated at 65 °C for 15 minutes.
Anwendung
XbaI is a restriction enxyme that cleaves DNA at the recognition site 5′-T/CTAGA-3′ to generate DNA fragments with 5′-cohesive termini.
Sonstige Hinweise
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Comment: Xba I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains). May cleave at secondary recognition sites under non-optimal conditions.
Physikalische Form
Solution in 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 0.1 mM PMSF, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.02% polydocanol at 4 °C
Ähnliches Produkt
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Stephanie L H Miller et al.
The Journal of biological chemistry, 279(32), 33528-33537 (2004-05-25)
The ubiquitin-interacting motif (UIM) is a short peptide motif with the dual function of binding ubiquitin and promoting ubiquitylation. This motif is conserved throughout eukaryotes and is present in numerous proteins involved in a wide variety of cellular processes including
A new specific endonuclease from Xanthomonas badrii..
Zain, B.S., and Roberts, R.J.,
Journal of Molecular Biology, 115, 244-255 (1977)
Miyuki Watabe et al.
The Ulster medical journal, 77(3), 168-174 (2008-10-30)
In Northern Ireland over the last 7 years, there is a mean of 41.9 laboratory reports per annum of human gastrointestinal infection (range 19-54) caused by Escherichia coli O157:H7. In the preceding years 1992-1996, reports were 5.4 per annum, whereas
E Malyguine et al.
Gene, 8(2), 163-177 (1980-01-01)
The specificity of XbaI, SalI, HhaI, PstI, BamHI and SstI is relaxed in the presence of an organic solvent, such as 20% glycerol or 8% dimethylsulfoxide (DMSO). This alteration, very pronounced in some cases, requires an excess of enzyme, varies
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.