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Merck

PIC0006

Sigma-Aldrich

Protease Inhibitor Cocktail

ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, Non-freezing solution

Synonym(e):

Protease inhibitor cocktail

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.28

product name

ReadyShield® Protease Inhibitor Cocktail, for use in tissue culture media, Non-freezing solution

Beschreibung

Tissue Culture Media

Form

ready-to-use solution

Qualität

200

Verpackung

pkg of 1 mL (in amber polypropylene vial)
pkg of 5 mL (in amber polypropylene vial)

Lagertemp.

−20°C

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Allgemeine Beschreibung

This product is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. It is designed to be used as a supplement to tissue culture medium for the prevention of secreted proteins degradation. The product has been found to be non-toxic after a 48 hours exposure to the following adherent cell lines: A431, CHO, COS, HepG2, and HeLa, and to following non-adherent cell lines: Jurkat and HL-60. This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0006 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P1860.

Leistungsmerkmale und Vorteile

  • Does not freeze in storage for quick, convenient use.
  • Inhibits serine, cysteine, aspartic proteases and aminopeptidases.
  • Tailored for use in tissue culture media.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Angaben zur Herstellung

It is recommended to determine the appropriate dilution for each specific cell line. This testing should begin with a dilution of at least a 200-fold to prevent deleterious effect of the solvent to cell growth.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Rechtliche Hinweise

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Artikel

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

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