P8398
Phospholipase D aus Kohl
Type IV, lyophilized powder, ≥100 units/mg solid
Synonym(e):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase, Phospholipase D from white cabbage
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About This Item
Empfohlene Produkte
Typ
Type IV
Form
lyophilized powder
Spezifische Aktivität
≥100 units/mg solid
Zusammensetzung
Protein, ~25%
Lagertemp.
−20°C
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Allgemeine Beschreibung
Phospholipase D (PLD) from cabbage comprises the C2 domain, PLD superfamily specific conserved regions (I–IV) with HxKx4Dx6GSxN (HKD) motif. Structurally, it is a barrel-shaped monomer bound to calcium ions.
Anwendung
Phospholipase D (PLD) is used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD is used in purification and kinetic studies.
The enzyme has been used for the preparation of Bodipy-phosphatidylcholine during the preparation of fluorescently labelled lipids.
Biochem./physiol. Wirkung
Phospholipase D from cabbage is widely used in biocatalytic transphosphatidylation reactions.
The amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD. It hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Einheitendefinition
One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.
Hinweis zur Analyse
Protein determined using biuret.
Inhibitor
Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Analysenzertifikate (COA)
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T T Allgyer et al.
Biochemistry, 18(24), 5348-5353 (1979-11-27)
Phospholipase D has been purified 680-fold from an acetone powder of savoy cabbage in an overall yield of 30%. The purification involves solubilization of the acetone powder in a Ca2+-containing buffer and subsequent ammonium sulfate fractionation. Gel filtration on Sephadex
Cindy Y Jao et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(36), 15332-15337 (2009-08-27)
Choline (Cho)-containing phospholipids are the most abundant phospholipids in cellular membranes and play fundamental structural as well as regulatory roles in cell metabolism and signaling. Although much is known about the biochemistry and metabolism of Cho phospholipids, their cell biology
Alexandra Lerchner et al.
Protein engineering, design & selection : PEDS, 19(10), 443-452 (2006-07-18)
In addition to hydrolysis of glycerophospholipids, phospholipases D (PLDs) catalyze the head group exchange. The molecular basis of this transphosphatidylation potential, which strongly varies for PLDs from different sources, is unknown hitherto. Recently, the genes of two PLD isoenzymes from
Susanne Stumpe et al.
The FEBS journal, 274(10), 2630-2640 (2007-04-25)
Phospholipases D play an important role in the regulation of cellular processes in plants and mammals. Moreover, they are an essential tool in the synthesis of phospholipids and phospholipid analogs. Knowledge of phospholipase D structures, however, is widely restricted to
Premysl Pejchar et al.
The New phytologist, 188(1), 150-160 (2010-07-16)
• Aluminium ions (Al) have been recognized as a major toxic factor for crop production in acidic soils. This study aimed to assess the impact of Al on the activity of phosphatidylcholine-hydrolysing phospholipase C (PC-PLC), a new member of the
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