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P7173

Sigma-Aldrich

Pyruvate Carboxylase from bovine liver

buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)

Synonym(e):

Pyruvate:CO2 ligase (ADP-forming)

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10 UNITS
1.010,00 €

1.010,00 €

Versandbereit am07. April 2025Details

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About This Item

CAS-Nummer:
9014-19-1
EC-Nummer:
6.4.1.1 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00132143
UNSPSC-Code:
12352204
NACRES:
NA.26

1.010,00 €

Versandbereit am07. April 2025Details

Form

buffered aqueous glycerol solution

Spezifische Aktivität

5-25 units/mg protein (BCA)

Konzentration

≥0.5 mg/mL

Fremdaktivität

lactic dehydrogenase ≤0.5%

Lagertemp.

−20°C

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Anwendung

Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.
The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.[1]

Biochem./physiol. Wirkung

Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA[2].

Einheitendefinition

One unit will convert 1.0 μmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30 °C.

Physikalische Form

Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.

Angaben zur Herstellung

Affinity purified

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000299813
0000259278
SLBF4710
058K7685
098H7481

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Philip Lee et al.
Hepatology (Baltimore, Md.), 57(2), 515-524 (2012-08-23)
The pathogenesis of type 2 diabetes is characterized by impaired insulin action and increased hepatic glucose production (HGP). Despite the importance of hepatic metabolic aberrations in diabetes development, there is currently no molecular probe that allows measurement of hepatic gluconeogenic
Eunsook S Jin et al.
Metabolism: clinical and experimental, 62(1), 152-162 (2012-09-18)
A three-day high-fat diet induces hepatic steatosis and hepatic insulin resistance in rats without altering fasting plasma glucose concentration or the rate of glucose production. However, as the nutrient profile available to the liver is substantially altered by a high-fat
H M White et al.
Journal of dairy science, 95(3), 1249-1256 (2012-03-01)
Expression of mRNA for pyruvate carboxylase (PC) is elevated at calving and during other physiological states when plasma NEFA concentrations are increased. The objective of this study was to determine the direct effects of fatty acids on expression of PC
Scott B Crown et al.
BMC systems biology, 6, 43-43 (2012-05-18)
¹³C-Metabolic flux analysis (¹³C-MFA) is a standard technique to probe cellular metabolism and elucidate in vivo metabolic fluxes. 13C-Tracer selection is an important step in conducting ¹³C-MFA, however, current methods are restricted to trial-and-error approaches, which commonly focus on an
Xiaoxia Yin et al.
Applied microbiology and biotechnology, 96(6), 1527-1537 (2012-06-09)
In previous research, a thiamine-auxotrophic yeast for alpha-ketoglutaric acid (KGA) overproduction was screened in our laboratory and named Yarrowia lipolytica WSH-Z06 (CCTCC no. M207143). However, the high concentration of by-products (mainly pyruvate) limited its application on an industrial scale. To
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