P4056

Platelet-Derived Growth Factor-BB from rat

PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): PDGF-BB
• MDL-Nummer: MFCD01094933
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 100
• Form: lyophilized powder
• Wirksamkeit: 1.62 ng/mL EC₅₀
• Qualität: endotoxin tested
• Mol-Gew.: ~30 kDa
• Verpackung: pkg of 5x50 μg; pkg of 50 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: ≤1 EU/μg
• UniProt-Hinterlegungsnummer: P31240
• Lagertemp.: −20°C
• Angaben zum Gen: rat ... Pdgfb(24628)

Beschreibung

Allgemeine Beschreibung

Platelet-derived growth factor (PDGF) is a 30 kDa cationic polypeptide and consists of two disulphide linked chains A and B. PDGF is expressed as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

Anwendung

Platelet-Derived Growth Factor-BB from rat has been used as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture and adult spinal cord neurons culture.

Biochem./physiol. Wirkung

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF activates smooth muscle relocalization and multiplication. PDGF mediated increased smooth muscle muscle proliferation might be associated with gene regulation. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.

Sonstige Hinweise

Dimer of the B chain of rat PDGF.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 2.5 mg bovine serum albumin.

Hinweis zur Analyse

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)