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Merck

MAK365

Sigma-Aldrich

Phenolic Compounds Assay Kit (Colorimetric)

sufficient for 200 colorimetric tests

Synonym(e):

Colorimetric Phenol Detection Kit

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 200 colorimetric tests

Anwendung(en)

cosmetics
food and beverages

Nachweisverfahren

colorimetric

Relevante Krankheit(en)

cancer; neurological disorders; cardiovascular diseases

Lagertemp.

−20°C

Allgemeine Beschreibung

Phenolic compounds are phytochemical secondary metabolites found abundantly in dietary and medicinal plants, vegetables and fruits. Major types of phytochemical phenolic compounds include simple phenolic acids (such as gallic acid and vanillic acid), flavonoids (such as catechin), stilbenoids, lignans and various highly complex polyphenols (proanthocyanidins and tannins). These compounds play an important role in plant defense against ultraviolet radiation, serve as a deterrent to herbivores and also act as signaling molecules in ripening and other plant growth processes. Both simple phenolic acids and complex polyphenols are found in high concentrations in foods and beverages such as berries, vegetables, cereals, coffee, tea and wine. Phenolic compounds, being antioxidants, have been increasingly studied in dietary sources, due to their protective effects against cardiovascular diseases, cancer and neurodegenerative diseases. Studies have also shown dietary polyphenols to possess antimicrobial, anti-inflammatory and anti-allergic properties.

Leistungsmerkmale und Vorteile

Compatible with high-throughput handling systems.

Eignung

Suitable for the measurement of phenolic compounds in fruits, vegetables, beverages (e.g. tea, wine, coffee), food products, plant extracts and natural or herbal products.

Prinzip

The Phenolic Compounds Assay Kit provides a quick, sensitive and selective method for measuring the total amount of phenolic compounds in various biological samples. In this assay, phenolic compounds couple with diazonium salts under alkaline conditions to form a stable diazo chromophore, detectable by absorbance at 480 nm. Unlike the classical Folin-Ciocalteu protocol for measuring phenolic compounds, this assay is not affected by non-phenolic reducing substances such as sulfites, reducing sugars or ascorbic acid. The assay is high-throughput adaptable and can detect concentrations of phenolic compounds as low as 0.02 mM catechin equivalents (CEs) from a variety of plant and food-based samples.

Piktogramme

FlameExclamation mark

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Eye Irrit. 2 - Flam. Liq. 2

Lagerklassenschlüssel

3 - Flammable liquids

Flammpunkt (°F)

49.5 °F

Flammpunkt (°C)

9.7 °C


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Rachele Francese et al.
PLoS neglected tropical diseases, 14(10), e0008713-e0008713 (2020-10-08)
The benefits of human milk are mediated by multiple nutritional, trophic, and immunological components, able to promote infant's growth, maturation of its immature gut, and to confer protection against infections. Despite these widely recognized properties, breast-feeding represents an important mother-to-child

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