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MAK317

Sigma-Aldrich

Sorbitol Dehydrogenase Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Nachweisverfahren

colorimetric

Relevante Krankheit(en)

endocrinological disorders, diabetes; gastrointestinal diseases

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage. Thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.
Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.

Leistungsmerkmale und Vorteile

Compatible with high-throughput handling systems.

Eignung

Suitable for determining sorbitol dehydrogenase (SDH) activity in a number of biological samples such as plasma, serum, urine, tissue, and culture media.

Prinzip

Sorbitol dehydrogenase assay is a non-radioactive, colorimetric assay. It is based on the reduction of the tetrazolium salt MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) in a NADH-coupled enzymatic reaction. The reduced form of MTT exhibits an absorption maximum at 565 nm and the increase in absorbance at 565 nm is directly proportional to the enzyme activity. This assay is based on a kinetic reaction. Sorbitol dehydrogenase (SDH) activity can be determined in a number of biological samples (e.g., plasma, serum, urine, tissue, and culture media).

Signalwort

Warning

Gefahreneinstufungen

Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

3 - Flammable liquids

Flammpunkt (°F)

75.2 °F - closed cup

Flammpunkt (°C)

24 °C - closed cup

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

The current state of serum biomarkers of hepatotoxicity.
Ozer J, et al.
Toxicology, 245(3), 194-205 (2008)
Luciana F Brito et al.
Applied microbiology and biotechnology, 104(11), 5095-5106 (2020-04-11)
Gene repression using the endonucleolytically deactivated dCas9 protein and sgRNAs (CRISPR interference or CRISPRi) is a useful approach to study gene functions. Here, we established CRISPRi in Paenibacillus sonchi genomovar Riograndensis SBR5, a plant growth promoting bacterium. CRISPRi system with
Pathophysiology of diabetic retinopathy.
Tarr J M, et al.
ISRN ophthalmology (2013)

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