There is no specific data available for cell media with this kit. Phenol red is excited at 440 nm, so it should not interfere in this case. However, it might elevate background fluorescence, so it may be advisable to choose a cell media that does not contain phenol red.
MAK272
High Sensitivity β-Hydroxybutyrate Fluorometric Assay Kit
sufficient for 100 fluorometric tests
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About This Item
UNSPSC-Code:
12161503
NACRES:
NA.25
Empfohlene Produkte
Anwendung(en)
cosmetics
food and beverages
Nachweisverfahren
fluorometric
Relevante Krankheit(en)
endocrinological disorders, diabetes; gastrointestinal diseases
Lagertemp.
−20°C
Allgemeine Beschreibung
β -Hydroxybutyrate (β-HB), also known as 3-hydroxybutyrate, is a ketone body primarily synthesized in the liver from the oxidation of fatty acids. Ketone body formation, ketogenesis, is elevated when blood glucose levels drop. Moderately elevated blood ketone bodies occur normally during fasting and prolonged exercise. During alcoholic or diabetic ketoacidosis, blood levels of β -Hydroxybutyrate can rise to abnormally high levels. β -Hydroxybutyrate can be used as a marker for hepatic energy metabolism.
Eignung
Suitable for use with serum, plasma, urine, and other biological fluids, as well as food products such as milk.
Prinzip
The High Sensitivity β-Hydroxybutyrate Assay Kit is a simple, sensitive, high-throughput adaptable method for quantifying β-hydroxybutyrate when sample quantities are limited or sub-ketogenic levels are suspected. In this assay, β-hydroxybutyrate is enzymatically oxidized generating a fluorometric signal (lex = 535 nm/lem = 587 nm), proportional to the amount of β-hydroxybutyrate present. The assay is sensitive to 4 mM of β-hydroxybutyrate in a variety of biological samples.
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10 - Combustible liquids
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Is it possible to use MAK272 to detect β-Hydroxybutyrate in phenol red-containing media?
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