M9317

Anti-MeCP2 antibody produced in rabbit

~0.6 mg/mL, affinity isolated antibody, buffered aqueous solution

• MDL-Nummer: MFCD02686372
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~75 kDa
• Speziesreaktivität: human
• Konzentration: ~0.6 mg/mL
• Methode(n): microarray: suitable; western blot: 0.5-1 μg/mL using nuclear extracts of the MCF7 breast carcinoma cell line.
• UniProt-Hinterlegungsnummer: P51608
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MECP2(4204) ; mouse ... Mecp2(17257) ; rat ... Mecp2(29386)

Beschreibung

Allgemeine Beschreibung

Methyl-CpG binding protein 2 (MeCP2) possesses a methyl-CpG-binding domain (MBD) and a transcriptional repression domain.

Immunogen

synthetic peptide corresponding to the C-terminus of human MeCP2 (amino acids 465-478) conjugated to KLH. The sequence is conserved in rat and mouse.

Biochem./physiol. Wirkung

MeCP2 can bind to a single symmetrically methylated CpG pair. It is also capable of binding to chromosomes at sites known to have methylated DNA. MeCP2 silences transcription by recruiting the histone deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor thus removing acetyl groups from histones and consequently silencing genes.

Methyl-CpG binding protein 2 (MeCP2) has been shown be crucial for brain development. It is part of the methyl-CpG-binding proteins, which are involved in repression of gene expression by binding to methylated DNA. Mutations in the gene encoding MeCP2 have been associated with Rett syndrome (RTT).

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)