M8177

Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) antibody, Mouse monoclonal

clone P38-TY, purified from hybridoma cell culture, buffered aqueous solution

• Synonym(e): Anti-MAP Kinase, p38, Activated, Monoclonal Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) antibody produced in mouse
• MDL-Nummer: MFCD00803556
• UNSPSC-Code: 12352203
• NACRES: NA.44

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: unconjugated
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: P38-TY, monoclonal
• Form: buffered aqueous solution
• Speziesreaktivität: rat, human, mouse
• Konzentration: ~2 mg/mL
• Methode(n): immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1 μg/mL using whole cell extract from a rat fibroblast cell line, Rat1, activated with sorbitol
• Isotyp: IgG2a
• UniProt-Hinterlegungsnummer: Q16539
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MAPK14(1432) ; mouse ... Mapk14(26416) ; rat ... Mapk14(81649)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) (mouse IgG2a isotype) is derived from the P38-TY hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The p38 mitogen-activated protein kinases (MAPK) comprises the stress-activated protein kinase (SAPK) 2b, SAPK3 and SAPK4 (also termed p38β-δ). All these kinases contain a glycine residue in their TXY(Thr−Xaa−Tyr motif) activation motif.

Spezifität

Reacts specifically with the active diphosphorylated form of p38 MAP kinase and its closely related isoforms. Does not recognize the non-phosphorylated and mono­phosphory­lated forms of the p38 MAP kinase molecule, or the non-phosphorylated, monophosphorylated and diphosphorylated forms of JNK and ERK-MAP kinases. The epitope recognized by the antibody contains the phosphorylated threonine and tyrosine residues within the regulatory site of active p38 MAP kinase (Thr180-Gly181-Tyr182).

Immunogen

Synthetic peptide HTDDEMpTGpYVATR corresponding to the phosphorylated form of p38 MAP kinase activation loop, coupled to KLH.

Anwendung

Monoclonal Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) antibody produced in mouse may be used in immunoblotting, enzyme linked immunosorbent assay (ELISA), immunostaining, western blotting, immunohistochemistry and in immunocytochemistry.

Biochem./physiol. Wirkung

The p38 mitogen-activated protein kinases (MAPK) are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residues determines the specificity of activation of the MAPKs, and is glutamic acid for extracellular-signal regulated kinase (ERK) (TEY), proline for c-Jun N-terminal kinases (JNK) and glycine for p38 MAPK. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. ERK generally transmits signals leading to cell proliferation, p38 MAPK and JNK are both mostly stress-responsive kinases3 and have been implicated in cell death in several cellular systems. Antibodies reacting specifically with activated p38 MAPK are useful tools in the study of the intracellular location of p38 MAPK enzymes, and in sorting out the signal transduction pathways of the MAPK superfamily.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Angaben zur Herstellung

From a culture supernatant of bioreactor grown hybridoma.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable