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Merck

L2157

Sigma-Aldrich

Laccase aus Rhus vernicifera

greener alternative

crude acetone powder, ≥50 units/mg solid

Synonym(e):

Benzoldiol:Sauerstoff-Oxidoreduktase

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

CAS-Nummer:
EC-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54

Form

crude acetone powder

Spezifische Aktivität

≥50 units/mg solid

Grünere Alternativprodukt-Eigenschaften

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Grünere Alternativprodukt-Kategorie

Lagertemp.

−20°C

SMILES String

[n]1(c(c(cc1C)C=O)C)CC

InChI

1S/C9H13NO/c1-4-10-7(2)5-9(6-11)8(10)3/h5-6H,4H2,1-3H3

InChIKey

NWDZDFOKSUDVJV-UHFFFAOYSA-N

Allgemeine Beschreibung

Wir verpflichten uns, Ihnen umweltfreundlichere Alternativprodukte anzubieten, die einen oder mehrere der „12 Prinzipien der Grünen Chemie“ erfüllen. Dieses Produkt wurde im Hinblick auf Energieeffizienz und Abfallvermeidung für die Verwendung in der Biobrennstoffzellen- und Celluloseethanol-Forschung verbessert. Weitere Informationen finden Sie in dem Artikel in Biofiles sowie unter Enzyme für die alternative Energieforschung.

Einheitendefinition

Eine Einheit erzeugt ein ΔA530 von 0,001 pro min bei pH 6,5 und 30 °C in einem 3-mL-Reaktionsvolumen unter Verwendung von Syringaldazin als Substrat.

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Analysenzertifikate (COA)

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Kunden haben sich ebenfalls angesehen

Mohammed Sherif et al.
Microbial biotechnology, 6(5), 588-597 (2013-07-03)
Multicopper oxidases can act on a broad spectrum of phenolic and non-phenolic compounds. These enzymes include laccases, which are widely distributed in plants and fungi, and were more recently identified in bacteria. Here, we present the results of biochemical and
Yu-Chan Zhang et al.
Nature biotechnology, 31(9), 848-852 (2013-07-23)
Increasing grain yields is a major focus of crop breeders around the world. Here we report that overexpression of the rice microRNA (miRNA) OsmiR397, which is naturally highly expressed in young panicles and grains, enlarges grain size and promotes panicle
Ngoc Han Tran et al.
Bioresource technology, 146, 721-731 (2013-08-21)
Many efforts have been made to understand the biodegradation of emerging trace organic contaminants (EOCs) in the natural and engineered systems. This review summarizes the current knowledge on the biodegradation of EOCs while having in-depth discussion on metabolism and cometabolism
Shanfa Lu et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(26), 10848-10853 (2013-06-12)
Laccases, as early as 1959, were proposed to catalyze the oxidative polymerization of monolignols. Genetic evidence in support of this hypothesis has been elusive due to functional redundancy of laccase genes. An Arabidopsis double mutant demonstrated the involvement of laccases
Jervian Johnson et al.
3 Biotech, 11(7), 360-360 (2021-07-24)
In this study, hydrolytic and oxidative activities of enzymes isolated from halophilic microbes were characterized and applied for biomass utilization. First, lipase from Micrococcus luteus, and peroxidase and laccase from Pseudoalteromonas phenolica and Pseudoalteromonas peptidolytica were selected and their catalytic

Protokolle

Laccase, a biocatalyst, oxidizes aromatic compounds with molecular oxygen reduction, making it useful in various applications.

Fragen

1-6 von 6 Fragen  
  1. What is the Department of Transportation shipping information for this product?

    1 Antwort
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Hilfreich?

  2. What is the pH optimum of Product L2157, Laccase from Rhus vernicifera?

    1 Antwort
    1. Our own assay is performed at pH 6.5.  One publication (Casella et al., J. Inorg. Biochem., 100, 2127-2139 (2006)) gives pH 6.5 as another value for the laccase from this species, with a range for enzyme activity of 4.3 to 9.

      Hilfreich?

  3. My solution of Product L2157, Laccase from Rhus vernicifera, isn't clear. Is this normal?

    1 Antwort
    1. Yes. L2157 is a crude acetone powder, and can contain other proteins, enzymes, and cellular debris.  A suspension may be prepared in 100 mM potassium phosphate buffer, pH 6.5, by homogenization with a hand-held homogenizer for 10 minutes. This preparation may then be centrifuged; the laccase activity will be present in the supernatant.

      Hilfreich?

  4. How stable are solutions of Product L2157, Laccase from Rhus vernicifera?

    1 Antwort
    1. Solutions should be prepared fresh, directly before use, as they are not stable as stock solutions.

      Hilfreich?

  5. What is the molecular weight (MW) of Product L2157, Laccase from Rhus vernicifera?

    1 Antwort
    1. Spira-Solomon and Solomon, J. Am. Chem. Soc., 109, 6421-6432 (1987) reports estimated molecular weights of 52 kDa (laccase 1), 55 kDa (laccase 2), and 70 kDa (laccase 3), as determined by gel filtration.

      Hilfreich?

  6. What is the temperature optimum of Product L2157, Laccase from Rhus vernicifera?

    1 Antwort
    1. Our own assay is performed at 30°C.  One publication (Casella et al., J. Inorg. Biochem., 100, 2127-2139 (2006)) gives 25°C as another value for the same enzyme from the same species.

      Hilfreich?

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