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Merck

K4002

Sigma-Aldrich

Krebs-Ringer Bicarbonate Buffer

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution

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About This Item

UNSPSC-Code:
12161702
NACRES:
NA.75

Qualitätsniveau

Form

powder

Methode(n)

cell culture | mammalian: suitable

pH-Wert

6.1-6.7

Löslichkeit

water: 9.5 g/L, clear

Lagertemp.

2-8°C

Allgemeine Beschreibung

Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.

Anwendung

Krebs-Ringer Bicarbonate Buffer has been used:

  • as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
  • in the pre-perifusion for islet viability and functionality studies
  • in pituitary bioassay

Menge

Formulated to contain 9.5 grams of powder per liter of medium.

Rekonstituierung

Supplement with 1.26 g/L sodium bicarbonate.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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European journal of immunology, 38(12), 3499-3507 (2008-11-11)
When leukocytes cross endothelial cells during the inflammatory response, membrane from the recently described lateral border recycling compartment (LBRC) is selectively targeted around diapedesing leukocytes. This "targeted recycling" is critical for leukocyte transendothelial migration. Blocking homophilic PECAM interactions between leukocytes
The Hypothalamic-Pituitary-Adrenal Axis Response to Stress in Mice Lacking Functional Vasopressin V1b Receptors
Stephen J L, et al.
Endocrinology, 148, 849?856-849?856 (2007)
Activation of G Protein-Coupled Receptor 43 in Adipocytes Leads to Inhibition of Lipolysis and Suppression of Plasma Free Fatty Acids
Hongfei G, et al.
Endocrinology, 149, 4519?4526-4519?4526 (2008)
Guangrui Yang et al.
PloS one, 7(8), e38117-e38117 (2012-08-18)
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Protokolle

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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