H8541

Transforming Growth Factor-β1 human

TGF-β1, Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture

• Synonym(e): TGF-β1
• MDL-Nummer: MFCD00166089
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 300
• Rekombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: ≤0.5 ng/mL EC₅₀
• Qualität: endotoxin tested
• Mol-Gew.: dimer 25 kDa (non-glycosylated)
• Verpackung: pkg of 5 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: ≤1 EU/μg
• UniProt-Hinterlegungsnummer: P01137
• Lagertemp.: -10 to -25°C
• Angaben zum Gen: human ... TGFB1(7040)

Beschreibung

Allgemeine Beschreibung

The TGFB1 gene is mapped to human chromosome 19q13.2 and codes for a 25 kDa peptide. TGF-ß1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer.

Anwendung

Transforming Growth Factor-β1 (TGF-β) human has been used :

• to study the expression of PDGFRB (Platelet derived growth factor receptor β) in association with transforming growth factor-β signaling.
• to stimulate lung fibrosis in human fetal lung fibroblast cell line in order to further measure the in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device.
• to study the effect of TGF-β on HTRA1 (high-temperature requirement A1)-affected Wnt target genes.

Biochem./physiol. Wirkung

TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non-neoplastic tissues release transforming growth factors and essentially all cells possess a specific TGF-β1 receptor. In general, cells of mesenchymal origin appear to be stimulated by TGF-β1; whereas, hepatocytes, T and B lymphocytes, keratinocytes, and many epithelial cells are inhibited by the peptide. TGF-β1 interacts with Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Fibroblast Growth Factor (FGF), and T Cell Growth Factor (T-CGF) either by enhancing or antagonizing their characteristic actions. TGF-β1 plays a fundamental role in tissue growth and differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation, and immune cell function. TGF-β1 is associated with the developmental and metastasis of colorectal cancer.

Transforming Growth Factor-β1 (TGF-β1) wird von vielen Zelltypen produziert, ist jedoch Berichten zufolge in Säugetierthrombozyten am stärksten konzentriert und dort in etwa der vierfachen Konzentration des TGF-β2 vorhanden.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5

Hinweis zur Analyse

The activity was determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Danger
• H-Sätze: H314
• P-Sätze: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Gefahreneinstufungen: Eye Dam. 1 - Skin Corr. 1B
• Lagerklassenschlüssel: 8A - Combustible corrosive hazardous materials
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable