H7414

Anti-HIP14 in Kaninchen hergestellte Antikörper

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-Huntingtin-Interacting Protein 14, Anti-zinc finger, DHHC domain containing 17 (ZDHHC17), HSPC294, Huntingtin interacting protein 3 (HIP3), Huntingtin interacting protein H (HYPH), KIAA0946
• MDL-Nummer: MFCD09265361
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~75 kDa (mouse); antigen ~78 kDa (human)
• Speziesreaktivität: mouse, canine, human, rat, bovine
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• Methode(n): western blot: 0.5-1 μg/mL using extracts of HEK-293T cells expressing human HIP14 and mouse brain extracts (P2 membrane fraction)
• UniProt-Hinterlegungsnummer: Q8IUH5
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ZDHHC17(23390) ; mouse ... Zdhhc17(320150)

Beschreibung

Allgemeine Beschreibung

Huntingtin interacting protein 14 (HIP14) is a palmitoyl transferase (PAT) neuronal type, localizes to the Golgi, and to vesicles in the cytoplasm. HIP14 mRNA is predominantly expressed in the brain.

Immunogen

synthetic peptide corresponding to amino acids 614-632 located at the C-terminus of human HIP14. This sequence is identical in mouse, rat, dog, and bovine HIP14.

Anwendung

Anti-HIP14 antibody produced in rabbit has been used in quantitative immunoblotting, immunohistochemistry.

Biochem./physiol. Wirkung

The interaction of huntingtin interacting protein 14 (HIP14) with huntingtin protein (htt) is inversely correlated to the polyQ length.. HIP14 along with htt is localized in striatum, medium spiny projection neurons. It is also associated neurons associated with Huntington disease (HD). HIP14 under expression in neurons reduces the clustering of postsynaptic density protein 95 (PSD-95) and glutamic acid decarboxylase 65-kilodalton isoform (GAD65). It has been suggested that HIP14 has a role in palmitoylation and in regulating the intracellular trafficking of multiple neuronal proteins. Decreased interaction between mutant htt and HIP14 could contribute to neuronal dysfunction in HD by perturbing normal intracellular transport pathways in neurons.

Zielbeschreibung

HIP14 (also known as zinc finger, DHHC domain containing protein 17 (ZDHHC17), huntingtin interacting protein H (HYPH), huntingtin interacting protein 3 (HIP3), HSPC294 and KIAA0946) is a novel htt interacting protein.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)