Direkt zum Inhalt
Merck
Alle Fotos(1)

Wichtige Dokumente

H6914

Sigma-Aldrich

Humanserum

(from male AB clotted whole blood), USA origin, sterile-filtered

Synonym(e):

Humanseren, Humanserum, Off-the-Clot, otc

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise

Größe auswählen

20 ML
110,00 €
100 ML
522,00 €

110,00 €

Check Cart for Availability
Alle Fotos(1)

Größe auswählen

Ansicht ändern
20 ML
110,00 €
100 ML
522,00 €

About This Item

MDL-Nummer:
MFCD00165829
UNSPSC-Code:
12352207
NACRES:
NA.71

110,00 €

Check Cart for Availability

Biologische Quelle

human

Qualitätsniveau

300

Sterilität

sterile-filtered

Zusammensetzung

Hemoglobin, ≤25 mg/dL

Ursprung

USA origin

Methode(n)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

Verunreinigungen

≤10 EU/mL Endotoxin

Versandbedingung

dry ice

Lagertemp.

−20°C

Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich

Verwandte Kategorien

Anwendung

Humanes AB-Serum wird für folgende Anwendungen eingesetzt: Gewebezüchtung; Transplantations- und Zelltherapieanwendungen zur Expansion mesenchymaler Stammzellen aus adipösem Gewebe oder mesenchymaler Stromazellen aus humanem Knochenmark; standardisierte Gewinnung und Transplantation limbaler epithelialer Stammzelltransplantate, Ex-vivo-Expansion von NK-Zellen aus peripherem Blut in Expansionsmedium für hematopoetische Stammzellen; Verbesserung der Kulturmethoden für humane Inselzellen vor der Transplantation.

Verpackung

Die Verpackung kann sich aufgrund einer Knappheit der Flaschen, die zum Verpacken dieses Produkts verwendet werden, bei verschiedenen Chargen unterscheiden. Das Vorhandensein oder Fehlen von Schrumpffolie um den Flaschendeckel beeinträchtigt die Integrität/Stabilität des Produkts nicht.

Warnhinweis

Jeder Spender wurde auf Hepatitis B und C sowie mittels ELISA auf Antikörper gegen das humane Immundefizienzvirus (HIV) getestet und als nicht reaktiv befunden. Produkte humanen Ursprungs sollten dennoch als potenziell infektiös betrachtet und dementsprechend gehandhabt werden.

Hinweis zur Analyse

Endotoxin-getestet

Sonstige Hinweise

Ein trübes Aussehen unserer Humanserumprodukte ist nicht ungewöhnlich. Dieses Produkt besteht aus gepooltem Serum. Eine Trübung zeigt an, dass das Serum Lipide enthält. Klare, lipidfreie Humanserumprodukte sind nicht erhältlich.

Haftungsausschluss

NUR FÜR FORSCHUNGSZWECKE. Dieses Produkt unterliegt in Frankreich den Vorschriften für den Gebrauch zu wissenschaftlichen Zwecken, auch bei der Ein- und Ausfuhr (Artikel L 1211-1 Absatz 2 des französischen Gesundheitsgesetzes). Der Käufer (d. h. der Endverbraucher) ist verpflichtet, eine Einfuhrgenehmigung des französischen Forschungsministeriums gemäß Artikel 1245-5-1 II. des französischen Gesundheitsgesetzes einzuholen. Mit der Bestellung dieses Produkts bestätigen Sie, dass Sie über die entsprechende Einfuhrgenehmigung verfügen.

Ähnliches Produkt

Produkt-Nr.
Beschreibung
Preisangaben

06693

Timestrip Plus™ -20 °C

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000298253
0000298253
0000298252
0000298252
0000343425

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

Suche nach COA

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Alejandro Bustamante et al.
Stroke, 48(9), 2419-2425 (2017-07-19)
Stroke diagnosis could be challenging in the acute phase. We aimed to develop a blood-based diagnostic tool to differentiate between real strokes and stroke mimics and between ischemic and hemorrhagic strokes in the hyperacute phase. The Stroke-Chip was a prospective
Laura Abraira et al.
Epilepsia, 61(10), 2244-2253 (2020-08-29)
Blood biomarkers have not been widely investigated in poststroke epilepsy. In this study, we aimed to describe clinical factors and biomarkers present during acute stroke and analyze their association with the development of epilepsy at long term. A panel of
Ankit Sinha et al.
Cell systems, 8(4), 345-351 (2019-04-15)
High-grade serous ovarian carcinoma (HGSC) is the most common and lethal subtype of gynecologic malignancy in women. The current standard of treatment combines cytoreductive surgery and chemotherapy. Despite the efficacy of initial treatment, most patients develop cancer recurrence, and 70%
Kwang Hoe Kim et al.
Analytica chimica acta, 882, 38-48 (2015-06-05)
Multiple reaction monitoring (MRM) is commonly used for the quantitative analysis of proteins during mass pectrometry (MS), and has excellent specificity and sensitivity for an analyte in a complex sample. In this study, a pseudo-MRM method for the quantitative analysis
Hien T Ngoc Le et al.
Micromachines, 11(9) (2020-08-23)
Amyloid beta (aβ) 1-42, a peptide that is 1-42 amino acids long, is a major component of senile plaques in the brains of patients with Alzheimer's disease. Aβ detection has become an essential antecedence to predict the declining mental abilities
Alle zugehörigen Dokumente anzeigen

Artikel

Serum for Cell and Tissue Culture FAQs

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

Fragen

1-9 von 9 Fragen  

  1. What is the best way to thaw this product? Once thawed, how long can we use it if kept in at 4 oC?

    1 Antwort

    1. The recommended storage temperature is -20°C. The following steps confirm the best way to thaw serum:

      1. Remove the serum bottles from the freezer and allow them to acclimate to room temperature for approximately 10 minutes.
      2. Place each container in a 30 to 37 °C water bath or incubator. Excessive temperatures will degrade heat-labile nutrients. If using a
      water bath, prevent the bottle caps from being completely submerged.
      3. Gently swirl or shake the bottles every 10 - 15 minutes until the serum is completely thawed.
      Please note: Multiple thaw/freeze cycles should be avoided as they will hasten serum nutrient degradation and can induce insoluble precipitate formation.

      Hilfreich?

  2. Is this serum manufactured under cGMP?

    1 Antwort

    1. This product is not manufactured under cGMP.

      Hilfreich?

  3. If the serum is being used to complete media for a cell thaw but then the cells are put through a DNA extraction rather than put into culture - would thawing a bottle of serum, aliquoting, and then re-freezing the serum be okay?

    1 Antwort

    1. Thawed serum should be mixed thoroughly before being added to cell culture medium. Repeated freeze-thaw cycles may significantly impact serum quality. It is recommended to aliquot the thawed serum for single use and store aliquots at -20 °C. If serum is stored between 2-8 °C, it should be used within 2-4 weeks.

      Hilfreich?

  4. DOES THIS PRODUCT CONTAIN OESTRADIOL

    1 Antwort

    1. The presence of oestradiol in this product is not determined. To see which compounds are tested for, refer to a sample Certificate of Analysis found at: https://www.sigmaaldrich.com/product/sigma/h6914#product-documentation

      Hilfreich?

  5. At which biosafety level should Human Serum H6914 be handled?

    1 Antwort

    1. Blood products should be treated and handled as potentially infectious. It is recommended to use human origin products from normal (non-diseased) donors at Biosafety Level 2 (BSL-2). Although donors may be tested for adventitious infections, as listed on the Certificate of Analysis for the product, no testing can cover all types of infection. Therefore, these products should be treated as potentially infectious and handled accordingly. Laboratory directors and principal investigators are responsible for performing a risk assessment and determining the biosafety level in accordance with applicable national and local regulations.

      Hilfreich?

  6. Human Serum (Product No. H6914 & Batch No. SLCF0424)의 사용기한은 CoA상에서 2023년 2월 까지인 것으로 확인됩니다. 혹시 해당 batch의 사용기한이 연장될 수도 있을까요?

    1 Antwort

    1. The shelf life is not extended for those product which are assigned a firm expiration date. No further testing is performed on these lots.

      Hilfreich?

  7. Are all your human donors consented?

    1 Antwort

    1. All protocols including donor consent are subject to the strict oversight of an Institutional Review Board (IRB) as governed by US law.

      Hilfreich?

  8. What is the Department of Transportation shipping information for this product?

    1 Antwort

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Hilfreich?

  9. What is the difference between human serum from AB plasma and  human serum from AB clotted whole blood?

    1 Antwort

    1. Product H6914 is prepared from whole blood that is allowed to clot, centrifuged and the serum removed. There is no additional processing required.Product H4522 is prepared from anticoagulated (sodium citrate) whole blood. This is centrifuged to pellet the cells (RBC, WBC, some platelets) and the plasma is removed. This plasma is then treated with calcium to activate the clotting cascade. The preparation is centrifuged again and the supernatant is removed. This product can also be called plasma derived serum.Product H6914 will contain more growth factors since it is allowed to spontaneously clot and there will be the release of growth factors from many of the cells (WBC, platelets) present.  The cells are removed before the plasma is clotted for Product H4522, so this plasma will have fewer growth factors present (more similar to what is circulating). Unless testing for growth factors, most researchers are looking for Product H6914.We offer both products heat inactivated.Product H5667 Human Serum Heat Inactivated, from male AB clotted whole bloodProduct H3667 Human Serum Heat Inactivated, from human male AB plasma

      Hilfreich?

Bewertungen

Kein Beurteilungswert

Aktive Filter

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.