The lyophilized powder may be reconstituted at 5 mg/mL in 0.02M sodium phoshate buffer or 0.45% sodium chloride containing 0.01% BSA. Solutions prepared at concentrations of at least 1 mg/ml may be stored in aliquots at -20°C for several months.
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H2126
Hyaluronidase aus Schaftestes
Type II, lyophilized powder, ≥300 units/mg solid
Synonym(e):
Hyaluronat-4-glycanhydrolase, Hyaluronoglucosaminidase
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100 MG
69,80 €
500 MG
203,00 €
1 G
353,00 €
5 G
1.410,00 €
69,80 €
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Größe auswählen
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100 MG
69,80 €
500 MG
203,00 €
1 G
353,00 €
5 G
1.410,00 €
About This Item
CAS-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54
69,80 €
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Empfohlene Produkte
Konjugat
conjugate (Glucosaminoglycan)
Typ
Type II
Form
lyophilized powder
Spezifische Aktivität
≥300 units/mg solid
Mol-Gew.
55 kDa
Lagertemp.
−20°C
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Allgemeine Beschreibung
Hyaluronidase is a hyaluronic acid-metabolizing enzyme.[1] It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.[2]
Anwendung
Hyaluronidase from sheep testes has been used:
- in flow cytometry[3]
- in the isolation of putative spermatogonial stem cells (SSCs)[4]
- to study the effects of magnetic stimulation in magnetic hydrogel properties[5]
- in immunohistochemistry to visualize collagen type II distribution[6][7]
- to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings[8]
- for the degeneration of hydrogels[9]
Biochem./physiol. Wirkung
Diese Enzyme spalten randomisiert β-N-acetylhexosamin-[1−4]-glycosidische Bindungen in Hyaluronsäure, Chondroitin und Chondroitin-Sulfaten.
Einheitendefinition
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
Physikalische Form
Lyophilized powder containing lactose
Sonstige Hinweise
Weitere Informationen über Enzyme für die Analyse komplexer Kohlenhydrate finden Sie unter www.sigma-aldrich.com/enzymeexplorer
Signalwort
Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Artikel
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Protokolle
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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Kann die Hyaluronidase H2126 in PBS gelöst werden um sie dann in kleinen Aliquoten bei -20 C zu lagern?
1 Antwort-
Hilfreich?
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H2126-100MG Hyaluronidase from sheep testes についてですが、溶解方法を教えてください。
1 Antwort-
This enzyme is soluble at 1 mg/mL in 20 mM sodium phosphate buffer (pH 7.0), with 77 mM sodium chloride and 0.1 mg/mL BSA at 1 mg/mL. For assay purposes, this product is prepared at 0.3 mg/mL solution in Phosphate Buffer. Heating may be required, Please see the link below to review the full assay protocol:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-hyaluronidase#preparationPlease see the link below to review the product datasheet for a bovine Hyaluronidase, product H3884. This information may also be applied to the ovine source material:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/739/183/h3884pis-ms.pdfHilfreich?
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