GEE70092Y

Shrimp Alkaline Phosphatase

Cytiva E70092Y, pack of 500 U

• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: shrimp
• Form: liquid
• Mol-Gew.: 155 kDa
• Verpackung: pack of 500 U
• Hersteller/Markenname: Cytiva E70092Y
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Shrimp alkaline phosphatase (SAP) has a high specific activity and is a heat-labile alkaline phosphatase.[1] It can be inactivated by a short rise in temperature to 65 °C. SAP is a 155,000Da homodimeric protein with dimensions of about 95 Å×65 Å×50 Å and mol. wt of 155,000.[1][2]
Completely and irreversibly inactivated in Tris buffers at pH 8.0-8.5 by heating for 15 min at 65°C. No further treatment is necessary.

Biochem./physiol. Wirkung

Shrimp alkaline phosphatase (SAP) catalyzes the in vitro dephosphorylation of DNA or deoxynucleotides (dNTPs).[3] The enzyme activity can be completely inhibited by ethylenediaminetetraacetic acid (EDTA), but the activity can be restored to a large degree by zinc.[4]

Shrimp alkaline phosphatase (SAP) catalyzes the in vitro dephosphorylation of DNA or deoxynucleotides (dNTPs).[1] The enzyme activity can be completely inhibited by ethylenediaminetetraacetic acid (EDTA), but the activity can be restored to a large degree by zinc.[2]

Leistungsmerkmale und Vorteile

• Removing 5′-phosphates from DNA and RNA.
• Easily inactivated by heat.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids