GE17-5132-03
Glutathione Sepharose™ 4 Fast Flow
Cytiva 17-5132-03, pack of 500 mL
Synonym(e):
Glutathione Column, Sepharose 4 Fast Flow
About This Item
Empfohlene Produkte
ligand
glutathione and 10-carbon linker arm
Verpackung
pack of 500 mL
Hersteller/Markenname
Cytiva 17-5132-03
Matrix
highly cross-linked 4% agarose
Durchschnittlicher Durchmesser
90 μm
cleaning
3-12
working range
3-12
Kapazität
>10 mg/mL binding capacity
Verwandte Kategorien
Allgemeine Beschreibung
Glutathione Sepharose™ 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.
Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.
Leistungsmerkmale und Vorteile
- Fast, one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- For scale-up due to high flow properties
- Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
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Artikel
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
Protokolle
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.
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