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GE17-0920-07

IMAC Sepharose™ High Performance

Cytiva 17-0920-07, pack of 100 mL

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About This Item

UNSPSC-Code:
41106500
NACRES:
NA.56
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Verpackung

pack of 100 mL

Hersteller/Markenname

Cytiva 17-0920-07

Matrix

highly cross-linked 6% agarose

Durchschnittliche Teilgröße

34 μm

cleaning in place

2-14(Medium uncharged with metal ions.)

Arbeitsbereich

3-12

Kapazität

≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

Verwandte Kategorien

Allgemeine Beschreibung

IMAC Sepharose™ High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Anwendung

Conveniently charge with your metal of choice. IMAC Sepharose™ FF is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2?, Zn2?, Ni2?, or Co2?); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose. Small bead size for high performance purification and high binding capacity.

Leistungsmerkmale und Vorteile

  • For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
  • Conveniently charge with your metal of choice
  • 34 μm bead size for high performance purification
  • High binding capacity

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Sepharose is a trademark of Cytiva

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

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Analysenzertifikate (COA)

Lot/Batch Number

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Artikel

Immobilisierte Metallchelat-Affinitätschromatographie (IMAC)

Trennen von Proteinen und Peptiden mit Affinität zu Metallionen durch Immobilisierte-Metallionen-Affinitätschromatographie.

Immobilized Metal Chelate Affinity Chromatography (IMAC)

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

Purification or Removal of Proteins and Peptides

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

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Protokolle

Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance

Protocol for Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance

Separation with Cytiva Products

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

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