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FA20

Supelco

Fructose-Assay-Kit

sufficient for 20 assays

Synonym(e):

Fructose-Testsatz

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1 KIT
295,00 €

295,00 €


Versandbereit am04. April 2025Details


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1 KIT
295,00 €

About This Item

EG-Nummer:
UNSPSC-Code:
12164500
NACRES:
NA.84

295,00 €


Versandbereit am04. April 2025Details


Bulk-Bestellung anfordernWeitere Informationen anfragen

Verwendung

sufficient for 20 assays

chemische Klasse(n) des Analyten

sugars (fructose)

Methode(n)

photometry: suitable

Anwendung(en)

food and beverages
general analytical

Lagertemp.

2-8°C

Anwendung

Dieses Kit dient der quantitativen enzymatischen Bestimmung von Frucose in Lebensmitteln oder anderen Proben. Fructose wird bei der Hexokinase-katalysierten Reaktion durch Adenosintriphosphat (ATP) phosporyliert. Fructose-6-phosphat wird durch Phosphoglucoseisomerase (PGI) zu Glucose-6-phosphat umgewandelt. Glucose-6-phosphat (G6P) wird anschliessend in Gegenwart von Nicotinamidadenindinucleotid (NAD) zu 6-Phosphogluconat oxidiert, wobei Glucose-6-phosphat-Dehydrogenase (G6PDH) als Katalysator fungiert. Während dieser Oxidation wird eine equimolare Menge von NAD zu NADH reduziert. Der damit einhergehende Anstieg der Absorption bei 340 nm ist direkt proportional zur anfänglichen Fruktose-Konzentration in der Probe.

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung

  • fructose standard 500 mg

  • glucose assay reagent 1 mL/vial

  • phosphoglucose isomerase 1 mL/vial

Ähnliches Produkt

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3


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Analysenzertifikate (COA)

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Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Official Methods of Analysis of the AOAC, 28-28 (1995)
Yifan Chen et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 36(3), 373-378 (2019-11-19)
Cholesteryl ester (CE) is an ester of cholesterol and fatty acid (FA). Plasma CE reflects complicated metabolisms of cholesterol, phospholipids, lipoproteins, and dietary FAs. An informatics approach could be useful for analysis of the CE species. In this study, two
Mengxuan Fang et al.
Analytical and bioanalytical chemistry, 412(10), 2339-2351 (2020-02-02)
Fatty acids are a major source of structural diversity within the lipidome due to variations in their acyl chain lengths, branching, and cyclization, as well as the number, position, and stereochemistry of double bonds within their mono- and poly-unsaturated species.
Chih-Yu Cheng et al.
FEBS letters, 589(20 Pt B), 3107-3112 (2015-09-04)
Laccase and borate-fructose complex were investigated by coincidence in a solid-state fermentation of Edenia sp. TS-76 under fructose oxidase screening. Laccase was purified to homogeneity with a 34-fold purification and 32% yield. Fructose had no significant effect on laccase activity
Beuter, H.O.
Methods of Enzymatic Analysis, 6, 321-321 (1985)

Fragen

1-3 von 3 Fragen  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 Antwort
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 Antwort
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Hilfreich?

  3. イネの根および葉のフルクトース含量を測定したいと思っています。こちらのキットを使えるでしょうか。また、商品の詳細(分析手順等)について教えていただけると幸いです。

    1 Antwort
    1. Yes, this kit would be suitable. Here is the link to the Technical Bulletin that describes the use of this product:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/111/939/fa20bul.pdf
      For roots and leaves, one would follow the Sample Preparation protocol for Solids. The plant tissue would need to be macerated in order to completely extract the Fructose into the water.

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