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D3812

Sigma-Aldrich

DirectLoad™ 50 bp DNA Step Ladder

ready-to-use marker for DNA electrophoresis

Synonym(e):

DirectLoad™ 50 bp DNA Marker, Ready-to-use 50 bp DNA Marker, Ready-to-use DirectLoad™ 50 bp DNA Marker, Ready-to-use DirectLoad™ 50 bp DNA Step Ladder

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About This Item

UNSPSC-Code:
41105335
NACRES:
NA.25

Qualitätsniveau

200

Form

(liquid)

Verwendung

sufficient for 100 loads

Eignung

suitable for electrophoresis (DNA)

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

Sigma′s DirectLoad™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Anwendung

DirectLoad™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Leistungsmerkmale und Vorteile

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Komponenten

Sigma′s DirectLoad™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Sonstige Hinweise

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Rechtliche Hinweise

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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FEMS microbiology letters, 307(2), 158-164 (2010-04-24)
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Bulkescher R, et al.
Genome Research, 27(10), 1752-1758 (2017)

Artikel

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Protokolle

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