PGF human cell line

94050342, IHW Number: 9318

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human blood
• Ethnizität: English
• Geschlecht: male
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblastoid
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice

Beschreibung

Allgemeine Beschreibung

Consanguineous: Yes

Homozygous: Yes

Beschreibung der Zelllinie

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA-Profil

A: 301
Cw: 7
B: 702
HLA DRB1: 1501
HLA DRB5: 101
HLA DRB6: 201
HLA DQA1: 102
HLA DQB1: 602
HLA DPA1: 1
HLA DPB1: 401

Serologisches Profil

HLA A: 3
HLA B: 7
Bw4/bw6: 6
HLA Dw: 2
HLA DR: 15
HLA DR51: 51
HLA DQ: 6
HLA DP: 4

Nährmedium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subkultur-Routine

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Haftungsausschluss

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.