Y13-259

89111412, Reactivity: Anti-p21 Protein, Harvey Murine Sarcoma Virus

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Rat x Rat Hybridoma
• ImmunoDon: Osborne-Mendel rat spleen
• Myelom: Y3Ag1.2.3
• Reaktivität: Anti-p21 Protein, Harvey Murine Sarcoma Virus
• Methode(n): cell culture | mammalian: suitable
• Isotyp: IgG1
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat Y3Ag1.2.3 x Rat Osborne-Mendel spleen

Beschreibung der Zelllinie

The antibody reacts to p21 encoded by Ha-MuSV v-ras gene. Cross-reacts to p21 encoded by Kirsten MuSV v-ras gene, p21 encoded by BALB-SV and 29kDa gag-ras fusion protein encoded by Ra-SV. Recognises p21 species in some uninfected rodent, carnivore and primate lines.

Immunogen

Ha-MuSV transformed syngeneic NRK cells

Nährmedium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells will semi-attach. Shake cells off or use trypsin to remove cells from flask

Sonstige Hinweise

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