CCRF-HSB-2

85112801, human blood, Lymphoblast

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: CCRF-HSB-2, 85112801, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• Biologische Quelle: human blood
• Verpackung: tube of 5 μg 85112801-DNA-5UG; pkg of vial of cells 85112801-1VL
• Wachstumsmodus: Suspension
• Karyotyp: 2n = 46
• Morphologie: Lymphoblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian acute lymphoblastic leukaemia

Beschreibung der Zelllinie

A T lymphoblastoid line obtained from the peripheral blood of an 11 year old Caucasian male with acute lymphoblastoid leukaemia which has been passaged eight times through new-born Syrian Hamsters. These may be grown to a high density in a spinner type suspension culture.

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18,19

Nährmedium

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 5-8x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 2-3x1,000,000 cells/ml.

Sonstige Hinweise

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