LTK-

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011432, mouse connective tissue, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: mouse connective tissue
• Verpackung: tube of 5 μg 85011432-DNA-5UG; pkg of vial of cells 85011432-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse C34/An connective tissue

Beschreibung der Zelllinie

Sub-line of BUdR resistant strain of the L-M mouse line (Sigma Catalogue number. 87032401), which in turn was derived from L929 (Sigma Catalogue number. 85011425). TK- cells cannot grow in HAT medium. Stable TK- mutants are only maintained if the cells are kept for several passages in growth medium containing BUdR.

Anwendung

Somatic cell hybridisation and gene transfer studies

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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