A9

85011426, mouse adipose tissue, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: A9, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 85011426
• Biologische Quelle: mouse adipose tissue
• Verpackung: tube of 5 μg 85011426-DNA-5UG; pkg of vial of cells 85011426-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Aneuploid
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse C3H/An areolar and adipose tissue

Beschreibung der Zelllinie

Derivative of L929 selected in azaguanine (3μg/ml). Partially resistant to azaguanine. A9 may be heterozygous for the ability to synthesise active inosinic acid phosphorylase.

Anwendung

Metabolic studies

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-5x10,000/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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