108CC5-BU-5

8062567, Mouse x Rat Hybridoma nerve, Neuronal

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: 108CC5-BU-5, 08062567, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• Biologische Quelle: Mouse x Rat Hybridoma nerve
• Verpackung: tube of 5 μg 08062567-DNA-5UG; pkg of vial of cells 08062567-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Neuronal
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse neuroblastoma x Rat glioma hybrid

Beschreibung der Zelllinie

A bromodeoxyuridine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid

Anwendung

Used in the generation of cell hybrids.

Nährmedium

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell pellet in fresh medium using the appropriate volume to achieve the correct seeding density.

Sonstige Hinweise

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