C7688

Chaperonin 60 from Escherichia coli

>95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder

• Synonym(e): Cpn60, GroEL
• MDL-Nummer: MFCD00163256
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: Escherichia coli
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli overproducing strain
• Assay: >95% (SDS-PAGE)
• Form: lyophilized powder
• Methode(n): electron microscopy: suitable; mass spectrometry (MS): suitable
• Eignung: passes test (Refolding (w/GroES))
• UniProt-Hinterlegungsnummer: P10809
• Lagertemp.: 2-8°C
• Angaben zum Gen: human ... HSPD1(3329)

Beschreibung

Allgemeine Beschreibung

Research area: Cell Signaling

Chaperonin 60 (GroEL) and chaperonin 10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles.

Anwendung

Chaperonin 60 from Escherichia coli has been used:

• in mass spectroscopy[1]
• in cryo-electron microscopy imaging as control for testing particle distribution[2]
• as standard in infrared spectrum measurements[3]
• as a protein sample for stability assessment and characterization studies using differential mobility analysis (DMA) and cryo-electron microscopy (cryo-EM).[4]

Biochem./physiol. Wirkung

Apart from its role in facilitating protein folding, chaperonin 60 (Cpn60) serves as an extracellular signaling protein, showing functional similarities to certain proinflammatory cytokines.[5] Furthermore, Cpn60 contributes to the inhibition of lipid accumulation and adipogenesis during the initial stages of cellular differentiation, thereby exerting anti-obesity effects.[6]

Verpackung

Package size based on protein content.

Physikalische Form

Lyophilized powder containing Tris buffer salts, potassium chloride, magnesium chloride, dithiothreitol, and trehalose as stabilizer.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)