C2979

Caspase 7 human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, ≥1,500 units/mg protein

• Synonym(e): CMH-1, ICE-LAP3, Mch-3
• MDL-Nummer: MFCD01865995
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• Form: buffered aqueous solution
• Spezifische Aktivität: ≥1,500 units/mg protein
• Mol-Gew.: 56 kDa by immunoblotting
• UniProt-Hinterlegungsnummer: P55210
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... CASP7(840)

Beschreibung

Biochem./physiol. Wirkung

Cleaves the substrates consisting of consensus sequence DEVD.

Member of the CED-3 subfamily, the effector caspases, with an essential role in the apoptotic process. Upon activation, the zymogen is translocated to the mitochondrial and microsomal fractions. It is responsible for the cleavage of cellular substrates that precipitate the morphological changes of apoptosis.

Member of the CED-3 subfamily, the effector caspases; upon activation, the zymogen is translocated to the mitochondrial and microsomal fractions.

Useful in screening caspase inhibitors, studying enzyme regulation, determining specificities of caspase substrates, as well as using as a positive control in caspase activity assays and Western blot analyses.

Einheitendefinition

One unit will hydrolyze 1.0 nmol of Ac-Asp-Glu-Val-Asp-AFC per minute at pH 7.5 at 25 °C.

Physikalische Form

Solution in HEPES, NaCl, DTT and sucrose, without carrier protein

Hinweis zur Analyse

Migrates as two major polypeptides (20 kDa and 10 kDa) in immunoblotting. Two minor polypeptides derived from the large subunit are also present.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)