C1345

Katalase aus Rinderleber

powder, suitable for cell culture, 2,000-5,000 units/mg protein

• Synonym(e): H₂O₂:H₂O₂ Oxidoreduktase
• CAS-Nummer: 9001-05-2
• EC-Nummer: 1.11.1.6 (BRENDA, IUBMB)
• EG-Nummer: 232-577-1
• MDL-Nummer: MFCD00081483
• UNSPSC-Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.75

Eigenschaften

• Biologische Quelle: bovine liver
• Produktlinie: BioReagent
• Form: powder
• Spezifische Aktivität: 2,000-5,000 units/mg protein
• Mol-Gew.: tetramer ~250 kDa
• Methode(n): cell culture | mammalian: suitable
• Isoelektrischer Punkt: 5.4
• UniProt-Hinterlegungsnummer: P00432
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
• InChIKey: NUVBSKCKDOMJSU-UHFFFAOYSA-N
• Angaben zum Gen: cow ... CAT(280743)

Beschreibung

Allgemeine Beschreibung

CAT (catalase) is an endogenous antioxidant enzyme, thus conferring protection to cells against damage by ROS (reactive oxygen species). In humans, this gene is localized to chromosome 11p13, which is composed of 12 introns and 13 exons.

Research Area: Cell Signaling

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Anwendung

Catalase from bovine liver is used for the following applications:

• Gel filtration
• Analytical filtration on Ultrogel and glycerol gradient centrifugation
• Size exclusion chromatography (HPLC)-HPLC analysis of estrogen receptor
• Enzyme assays
• Alcohol oxidase activity

Katalase wirkt als natürliches Antioxidans zur Untersuchung der Rolle reaktiver Sauerstoffspezies bei der Genexpression und Apoptose. Sie wird auch zum Schutz vor oxidativen Schäden an Proteinen, Lipiden und Nukleinsäuren eingesetzt. In der Industrie werden Katalasen verwendet, um in Milch und Käse oder beim Bleichen von Textilien zugesetztes Wasserstoffperoxid zu entfernen und dessen positive Auswirkungen auf die Lebensfähigkeit von DNA-Reparaturmutanten von E. coli zu untersuchen.

Biochem./physiol. Wirkung

Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H₂O₂ molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.

This product doesn not need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

Vorsicht

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Einheitendefinition

One unit will decompose 1.0 μmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

Angaben zur Herstellung

This product is a powder suitable for cell culture with activity at 2,000-5,000 units/mg. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)