Pericyte isolation generally starts with enzymatic digestion of tissue with Trypsin EDTA, usually followed by the isolation of microvessel fragments via successive filtration steps. Pure pericyte cultures can be obtained through either positive or negative immuno-selection via the use of magnetic beads or flow cytometry using the antibody markers above.
C-12981
Human Pericytes from Placenta (hPC-PL)
500,000 proliferating cells
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About This Item
UNSPSC-Code:
41106514
NACRES:
NA.81
Empfohlene Produkte
Biologische Quelle
placenta
Verpackung
pkg of 500,000 cells
Morphologie
( pericytes)
Methode(n)
cell culture | mammalian: suitable
Versandbedingung
ambient
Lagertemp.
room temp
Allgemeine Beschreibung
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Ursprung der Zelllinie
Placenta
Anwendung
Human Pericytes are isolated from human placenta tissue and provided in a proliferating format. Pericytes are multipotent mesenchymal-like cells found in association with small blood vessel walls. They are important for angiogenesis, the structural integrity of the microvasculature, and blood flow regulation. However, they can also develop into malignant tumors called hemangiopericytomas. To date, pericytes have demonstrated the ability to differentiate into adipocytes, osteoblasts, fibroblasts, smooth muscle cells, and phagocytes (macrophages).
Qualität
Rigid quality control tests are performed for each lot of Pericytes. The cells are tested for morphology, adherence rate, and viability. Furthermore, they are characterized by flow cytometric analysis for a comprehensive panel ofmarkers, e.g. CD31, CD34, CD105, and CD146. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).
Warnhinweis
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Subkultur-Routine
Click here for more information.
Sonstige Hinweise
Recommended Plating Density: 4000 cells per cm2Tested markers: CD146+, CD105+, CD31-, CD34-
Empfohlene Produkte
Recommended Primary Cell Culture Media:Link
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Artikel
What are pericytes? What are common pericyte markers? Are pericytes mesenchymal stem cells? Learn more about pericytes in our FAQ page. Explore over 350 PromoCell products.
Primary human pericytes and cell culture media optimized for the in vitro cultivation of human pericytes from all tissues. Protocols for handling, cell passaging, media, and product use.
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Protokolle
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Verwandter Inhalt
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How are pericytes isolated?
1 Antwort-
Hilfreich?
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What markers are used to characterize pericytes?
1 Antwort-
Pericytes are positive for CD146, PDGFRβ, αSMA, CD13, NG2, Desmin, and negative for CD31, CD34, CD45, and CD56.
Hilfreich?
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How long do pericytes grow in culture?
1 Antwort-
PromoCell’s human pericytes derived from microvessels of the human placenta are cryopreserved at the end of secondary culture (P1) and after thawing are considered P2. The number of populations doublings is not determined for each individual cell lot, however they are guarenteed to be grown for at least 15 population doublings.
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