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Merck

B7632

Sigma-Aldrich

N-Benzoyl-Phe-Val-Arg-p-Nitroanilid -hydrochlorid

chromogenic, protease substrate, ≥98% (TLC), powder

Synonym(e):

N-Benzoyl-L-phenylalanyl-L-valyl-L-arginin-4-nitro-anilid -hydrochlorid

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About This Item

Empirische Formel (Hill-System):
C33H40N8O6 · HCl
CAS-Nummer:
Molekulargewicht:
681.18
Beilstein:
3027332
MDL-Nummer:
UNSPSC-Code:
12352204
PubChem Substanz-ID:
NACRES:
NA.32

product name

N-Benzoyl-Phe-Val-Arg-p-Nitroanilid -hydrochlorid, protease substrate

Assay

≥98% (TLC)

Form

powder

Löslichkeit

methanol: 20 mg/mL, clear, colorless to light yellow

Lagertemp.

−20°C

SMILES String

Cl.CC(C)[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)c2ccccc2)C(=O)N[C@@H](CCCNC(N)=N)C(=O)Nc3ccc(cc3)[N+]([O-])=O

InChI

1S/C33H40N8O6.ClH/c1-21(2)28(40-31(44)27(20-22-10-5-3-6-11-22)39-29(42)23-12-7-4-8-13-23)32(45)38-26(14-9-19-36-33(34)35)30(43)37-24-15-17-25(18-16-24)41(46)47;/h3-8,10-13,15-18,21,26-28H,9,14,19-20H2,1-2H3,(H,37,43)(H,38,45)(H,39,42)(H,40,44)(H4,34,35,36);1H/t26-,27-,28-;/m0./s1

InChIKey

PYVSMZDQQUZGPF-JAQKLANPSA-N

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Allgemeine Beschreibung

N-Benzoyl-Phe-Val-Arg-p-nitroanilide is a chromogenic protease substrate.

Anwendung

  • N

  • -Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride has been used: as a substrate: for trypsin-like enzyme in the soluble and particulate fractions of the hyphae
  • for the thrombin, recombinant and native batroxobin from snake venom
  • for fibrinolytic enzyme aprE2 in amidolytic activity assay

Verpackung

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrate

A substrate for trypsin, thrombin and reptilase.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

[Clinical evaluation of prothrombin assay using chromogenic peptide substrates (author's transl)].
Y Akiyama et al.
Rinsho byori. The Japanese journal of clinical pathology, 28(11), 1131-1135 (1980-11-01)
S Rosén
Hamostaseologie, 25(3), 259-266 (2005-08-23)
Chromogenic peptide substrates were developed more than 30 years ago. Although the use of chromogenic substrate methods in coagulation and fibrinolysis diagnostics was not as rapidly implemented as initially believed, they are now well established for several analytes such as
Anusorn Tinyou et al.
Acta tropica, 204, 105288-105288 (2019-12-08)
Gnathostoma spinigerum is a causative agent of human gnathostomiasis and infects people residing in endemic areas as well as travelers. Cutaneous and visceral larval migrants cause clinical manifestations, resulting in severe morbidity and mortality. To survive in hosts, these parasites
Milton Erthal et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 152(1), 54-59 (2008-10-14)
The production of enzymes and the colonization of leaves by Leucoagaricus gongylophorus were investigated to further understand the digestive interactions of leaf-cutting ant colonies. The enzymes detected were indicative of a saprophytic origin of this fungus, producing all the enzymes
I J Mackie et al.
Thrombosis research, 28(4), 499-507 (1982-11-15)
The Factor VIII content of Factor IX concentrates was investigated by agarose gel electrophoresis which removed the interfering effects of stabilisers and proteolytic enzyme inhibitors. Factor VIII coagulant activity (FVIII C) as measured by clotting and amidolytic methods correlated well

Protokolle

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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