If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
Wichtige Dokumente
T9285
Tris-EDTA-Puffer -Lösung
100X concentrate for Northern and Southern blotting
Synonym(e):
Tris-EDTA-Waschpuffer, TE-Puffer -Lösung
Größe auswählen
About This Item
Empfohlene Produkte
Qualität
for molecular biology
Qualitätsniveau
Sterilität
0.2 μm filtered
Form
liquid
pH-Wert
7.8-8.2 (1 × in water)
Anwendung(en)
sample preparation
Fremdaktivität
DNase, RNase, none detected
Lagertemp.
room temp
SMILES String
NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChIKey
VLEIUWBSEKKKFX-UHFFFAOYSA-N
Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich
Allgemeine Beschreibung
Anwendung
- Isolierung, Resuspendierung oder Lagerung von DNA und RNA für die PCR- oder qPCR[1][2]
- Gelelektrophorese, einschließlich Northern und Southern Blotting[3]
- Bakterienzellen, für den AmpC-Scheiben-Test, zur Messung von aus Zellen freigesetzten β-Lactamasen[4]
- Zur Antigendemaskierung in der Immunhistochemie[3]
Komponenten
Sonstige Hinweise
Ähnliches Produkt
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
nwg
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Hier finden Sie alle aktuellen Versionen:
Analysenzertifikate (COA)
Die passende Version wird nicht angezeigt?
Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
-
How can I determine the shelf life / expiration / retest date of this product?
1 Antwort-
Hilfreich?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 Antwort-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Hilfreich?
-
-
Can this Tris-EDTA buffer solution be used in an IHC protocol for antigen retrieval?
1 Antwort-
This product has not been tested specifically for antigen retrieval. The researcher would have to determine suitability. Please see the link below to product E1161. This product is a prepared 10X solution formulated specifically for retrieval and may be better suited the specified application:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/374/855/e1161pis.pdfHilfreich?
-
-
What is the Department of Transportation shipping information for this product?
1 Antwort-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Hilfreich?
-
-
What is the pH and color of Product T9285, Tris-EDTA buffer solution?
1 Antwort-
The pH and color information is available on the specification sheet as well as lot specific Certificate of Analysis.
Hilfreich?
-
-
What does 'for molecular biology' mean?
1 Antwort-
The phrase `for molecular biology' means that all lots of this buffer have been tested for DNase and RNase activity, and that no activity was detected.
Hilfreich?
-
-
What are the final concentrations of Tris and EDTA when Product T9285 is diluted to 1:100 to make it 1X?
1 Antwort-
When diluted 1:100, the solution is 10mM TRIS with 1mM EDTA.
Hilfreich?
-
-
What is the shelf life of Product T9285, Tris-EDTA buffer solution?
1 Antwort-
This buffer solution has a recommended retest date of three years. This information is available on the Specification Sheet, and on the lot-specific Certificates of Analysis.
Hilfreich?
-
Aktive Filter
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.