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T9285

Sigma-Aldrich

Tris-EDTA-Puffer -Lösung

100X concentrate for Northern and Southern blotting

Synonym(e):

Tris-EDTA-Waschpuffer, TE-Puffer -Lösung

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100 ML
52,70 €

52,70 €


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100 ML
52,70 €

About This Item

MDL-Nummer:
UNSPSC-Code:
41105319
PubChem Substanz-ID:
NACRES:
NA.25

52,70 €


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Qualität

for molecular biology

Qualitätsniveau

Sterilität

0.2 μm filtered

Form

liquid

pH-Wert

7.8-8.2 (1 × in water)

Anwendung(en)

sample preparation

Fremdaktivität

DNase, RNase, none detected

Lagertemp.

room temp

SMILES String

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChIKey

VLEIUWBSEKKKFX-UHFFFAOYSA-N

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Allgemeine Beschreibung

Tris-EDTA-Puffer wird häufig in der Molekularbiologie zur Rekonstitution und Lagerung vieler ausgefällter Biomoleküle, einschließlich DNA und RNA, verwendet. Tris-HCl ist ein häufig verwendeter Puffer, und EDTA ist ein Metallchelatbildner. TE-Puffer solubilisiert Nukleinsäuren und schützt gleichzeitig die Moleküle vor dem Abbau. Er wird zur Isolierung von Nukleinsäuren verwendet, zum Beispiel vor der Nukleinsäure-Gelelektrophorese und der Northern- oder Southern-Blot-Hybridisierung.

Anwendung

Anwendungsgebiete des TE-Puffers:
  • Isolierung, Resuspendierung oder Lagerung von DNA und RNA für die PCR- oder qPCR[1][2]
  • Gelelektrophorese, einschließlich Northern und Southern Blotting[3]
  • Bakterienzellen, für den AmpC-Scheiben-Test, zur Messung von aus Zellen freigesetzten β-Lactamasen[4]
  • Zur Antigendemaskierung in der Immunhistochemie[3]

Komponenten

TE-Puffer enthält 1 M Tris-HCl (pH-Wert etwa 8,0) mit 0,1 M EDTA.

Sonstige Hinweise

Füllen Sie bitte dieses Formular aus, um weitere Informationen zu unserem Sortiment an Biochemikalien zu erhalten.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

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Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Kashif Asghar et al.
Experimental and therapeutic medicine, 9(3), 901-904 (2015-02-11)
Indoleamine 2,3-dioxygenase (IDO) is an immunoregulatory enzyme. It plays a key role in various malignancies, infection and autoimmune diseases. IDO induces immunosuppression through the depletion of tryptophan and its downstream metabolites. Hepatitis C virus (HCV) has infected more than 12
AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases.
Black JA, et al.
Journal of Clinical Microbiology, 43, 3110-3113 (2005)
Shiraz Tyebji et al.
Cell reports, 31(4), 107573-107573 (2020-04-30)
Germline epigenetic factors influence transgenerational inheritance of behavioral traits upon changes in experience and environment. Immune activation due to infection can also modulate brain function, but whether this experience can be passed down to offspring remains unknown. Here, we show
Sarah Bøje et al.
Immunology and cell biology, 94(2), 185-195 (2015-08-14)
Chlamydia is the most widespread sexually transmitted bacterial disease and a prophylactic vaccine is highly needed. Ideally, this vaccine is required to induce a combined response of Th1 cell-mediated immune (CMI) response in concert with neutralizing antibodies. Using a novel
Jennifer A Black et al.
Journal of clinical microbiology, 43(7), 3110-3113 (2005-07-08)
Although plasmid-mediated AmpC beta-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. These enzymes are typically produced by isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. and are

Fragen

1-8 von 8 Fragen  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 Antwort
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 Antwort
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Hilfreich?

  3. Can this Tris-EDTA buffer solution be used in an IHC protocol for antigen retrieval?

    1 Antwort
    1. This product has not been tested specifically for antigen retrieval. The researcher would have to determine suitability. Please see the link below to product E1161. This product is a prepared 10X solution formulated specifically for retrieval and may be better suited the specified application:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/374/855/e1161pis.pdf

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  4. What is the Department of Transportation shipping information for this product?

    1 Antwort
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  5. What is the pH and color of Product T9285, Tris-EDTA buffer solution?

    1 Antwort
    1. The pH and color information is available on the specification sheet as well as lot specific Certificate of Analysis.

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  6. What does 'for molecular biology' mean?

    1 Antwort
    1. The phrase `for molecular biology' means that all lots of this buffer have been tested for DNase and RNase activity, and that no activity was detected.

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  7. What are the final concentrations of Tris and EDTA when Product T9285 is diluted to 1:100 to make it 1X?

    1 Antwort
    1. When diluted 1:100, the solution is 10mM TRIS with 1mM EDTA.

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  8. What is the shelf life of Product T9285, Tris-EDTA buffer solution?

    1 Antwort
    1. This buffer solution has a recommended retest date of three years. This information is available on the Specification Sheet, and on the lot-specific Certificates of Analysis.

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