RNB200

GenElute^™ Total RNA Purification Maxi Kit

• UNSPSC-Code: 41105600
• NACRES: NA.52

Eigenschaften

• Grünere Alternativprodukt-Eigenschaften: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• Grünere Alternativprodukt-Kategorie: Aligned,
• Versandbedingung: ambient
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

Sigma′s GenElute^™ Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

• Isolate true total RNA including siRNA and microRNA
• No phenol extractions
• Purify high-quality RNA in 40 minutes
• Isolate total RNA from a wide variety of sources

Anwendung

• Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
• RT-PCR for both large mRNA and small RNA including miRNA
• Expression profiling
• Next Generation Sequencing for RNA and miRNA
• miRNA from plasma for discovery
• microRNA cloning and amplification
• PCR-based virus detection
• PCR-based viable bacteria detection
• Northern blotting
• RNase protection
• Primer extension
• miRNA array
• RNA-Seq
• Nanostring
• Fluidigm
• Droplet/Digital PCR

Leistungsmerkmale und Vorteile

• Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
• Fast and easy processing - Rapid spin-column format allows for the processing of 4 samples in under 40 minutes.
• No phenol:chloroform extractions – Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform.
• Isolate total RNA from a wide variety of sources – Total RNA can be isolated from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses using this kit.
• Isolate inhibitor-free RNA suitable for downstream applications - Purified RNA can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array analysis requiring the use of intact RNA
• Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
• Fast and easy processing - Rapid spin-column format allows for the processing of 4 samples in under 40 minutes.
• No phenol:chloroform extractions – Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform.
• Isolate total RNA from a wide variety of sources – Total RNA can be isolated from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses using this kit.
• Isolate inhibitor-free RNA suitable for downstream applications - Purified RNA can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array analysis requiring the use of intact RNA

Einheitendefinition

Preps

Sonstige Hinweise

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Rechtliche Hinweise

GenElute is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Piktogramme: GHS05,GHS07,GHS09
• Signalwort: Danger
• H-Sätze: H302 + H332,H314,H410
• P-Sätze: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C
• Zusätzliche Gefahrenhinweise: EUH032
• Lagerklassenschlüssel: 8A - Combustible corrosive hazardous materials