DAPI is suitable for staining bacterial chromosomes, including those of E. coli.
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D9542
DAPI
DAPI
for nucleic acid staining
Synonym(e):
4′,6-Diamidin-2-phenylindol -dihydrochlorid, 2-(4-Amidinophenyl)-6-indolcarbamidin -dihydrochlorid, DAPI -dihydrochlorid
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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1 MG
15,70 €
5 MG
66,40 €
10 MG
113,00 €
50 MG
454,00 €
Alle Fotos(6)
Größe auswählen
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1 MG
15,70 €
5 MG
66,40 €
10 MG
113,00 €
50 MG
454,00 €
About This Item
Empirische Formel (Hill-System):
C16H15N5 · 2HCl
CAS-Nummer:
Molekulargewicht:
350.25
Beilstein:
4894417
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352200
PubChem Substanz-ID:
NACRES:
NA.52
Empfohlene Produkte
Qualität
Molecular Biology
for molecular biology
Assay
≥98% (HPLC)
Form
powder
Methode(n)
transfection: suitable
Löslichkeit
H2O: 20 mg/mL
PBS: insoluble
ε (Extinktionskoeffizient)
30 at 263 nm in H2O at 1 mM
Fluoreszenz
λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
Eignung
suitable for fluorescence
Lagertemp.
2-8°C
SMILES String
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChIKey
FPNZBYLXNYPRLR-UHFFFAOYSA-N
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Allgemeine Beschreibung
DAPI (4′,6-Diamidino-2-phenylindoldihydrochlorid) ist ein zelldurchdringender, fluoreszierender Farbstoff, der an DNA bindet.
Möglicherweise ist Wärme oder eine Ultraschallbehandlung erforderlich. Bei Lagerung bei Raumtemperatur oder bei 4 °C im Dunkeln sollten die Lösungen 2 bis 3 Wochen lang stabil sein.
Anwendung
DAPI ist um ein Vielfaches empfindlicher als Ethidiumbromid zur DNA-Färbung in Agarosegelen. Es kann für sogenanntes Photo-Footprinting von DNA verwendet werden, zum Nachweis gebundener Sonden in Blotting-Anwendungen durch gezielte Visualisierung des doppelsträngigen Komplexes, als auch um Änderungen in der DNA zu untersuchen und um den DNA-Gehalt während der Apoptose mittels Durchflusszytometrie zu bestimmen. DAPI-Färbung ist eine sensitive und spezifische Nachweismethode für Mykoplasmen.
DAPI ist um ein Vielfaches sensitiver als Ethidiumbromid für die Färbung von DNA in Agarosegelen.
Geeignet für
DAPI wird verwendet:
- DNA-Färbung in Agarosegelen
- Analyse von DNA-Veränderungen während der Apoptose
- Nachweis von Mykoplasmen
- Photo-Footprinting von DNA
- Immunfluoreszenzfärbung von Zellen
DAPI wird verwendet:
- zur schnellen Überwachung von mikrobiologischen Kontaminationen[1]
- bei der Chromosomenbänderungstechnik[2]
- zum Nachweis von apoptotischen Zellen[3]
- bei der Fluoreszenzmikroskopie zum Nachverfolgen der DisA (DNA-Integritäts-Scan-Protein)-Bewegung auf Bacillus subtilis-DNA[4]
- zum Färben von ausgereiften Pollenkörnern (0,5 mg/ml)[5]
Biochem./physiol. Wirkung
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Vorsicht
vor Licht schützen
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Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
Zielorgane
Respiratory system
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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In response to DNA damage, cells activate checkpoint signaling cascades to control cell-cycle progression and elicit DNA repair in order to maintain genomic integrity. The sensing and repair of lesions is critical for Bacillus subtilis cells entering the developmental process
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Can this product be used to stain bacteria (E.coli) chromosome?
1 Antwort-
Hilfreich?
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What is the working dilution of DAPI that should be used to stain the nuclei of human cells?
1 Antwort-
Typical working concentration of DAPI used in most applications is 0.1μg/mL-10μg/mL. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/774/984/d9564pis.pdfPlease see the link below to review a sample protocol from Cold Spring Harbor:
https://cshprotocols.cshlp.org/content/2011/1/pdb.prot5556.longHilfreich?
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what kind of the water used to dissolve DAPI? and what's the temperature should be dissolve the water?
1 Antwort-
This product is soluble at 20 mg/mL in water at ambient temperature. No specific water type or treatment is required. Deionized water is typically used for laboratory applications.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdfHilfreich?
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Can the stain concentration be used for staining fish scales and any animal cells for ICC?
1 Antwort-
Yes, DAPI can be used to stain fish scale nuclei. While specific data regarding the concentration and duration for staining fish scale nuclei is not available, it is suggested to start with a working concentration of around 0.5 mg/ml and optimize the staining for each specific cell type, as different cells may uptake the dye at varying rates. It is also advisable to try staining at both higher and lower concentrations. For staining mammalian cells, lower concentrations are typically used.
Hilfreich?
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What is the suitable DAPI for immunofluorescence staining of free-floating rat brain sections?
1 Antwort-
Several DAPI products are suitable for staining free-floating rat brain sections. The same DAPI used for staining mycoplasma, cultured cells, or other cell types can be used for this purpose. Any of the DAPI products listed on the website should be suitable for this use.
Hilfreich?
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What is the suggested concentration for product D9542-1MG?
1 Antwort-
The suggested concentration for product D9542-1MG, DAPI, is in the range of 0.5 to 5 ug/ml.
Hilfreich?
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肝細胞を染める染色プロトコルを教えてください。
1 Antwort-
Please see the link below to review the product datasheet, which includes basic protocols:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdfA protocol specifically for hepatocyte staining is not available. However, the publication shown at the link below may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671579/Hilfreich?
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What is the Department of Transportation shipping information for this product?
1 Antwort-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Hilfreich?
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How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be stored once in solution, and for how long would it be good?
1 Antwort-
Solutions stored in the dark at room temperature have been stable for 2-3 weeks, but solutions exposed to light for the same time period have shown considerable degradation. At 1 mg/mL, solutions are stable in the dark at 2-8 °C for several weeks. For long-term storage, the solutions can be divided into aliquots and stored at -20 °C.
Hilfreich?
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How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be dissolved?
1 Antwort-
This product is soluble in water at 20 mg/mL, but may require heat. DAPI does not dissolve readily in PBS, 0.1 M phosphate, or 0.1 M Tris (pH 7).
Hilfreich?
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