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BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

Synonym(e):

Competent E. coli cells

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About This Item

UNSPSC-Code:
41106202
NACRES:
NA.85
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Produktbezeichnung

BL21(DE3) Chemically Competent Cells, for protein expression

Biologische Quelle

Escherichia coli

Qualität

for molecular biology

Wachstumsmodus

adherent or suspension

Morphologie

rod shaped

Methode(n)

microbiological culture: suitable

Zelltransformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

Versandbedingung

dry ice

Lagertemp.

−70°C

Allgemeine Beschreibung

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Anwendung

The E. coli strain BL21 (DE3) has been used:

  • to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.[1]
  • BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.[2]
  • to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.[3][1]

Leistungsmerkmale und Vorteile

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Komponenten

  • BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

Lagerklassenschlüssel

10 - Combustible liquids


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Romana Koszagova et al.
Microbial cell factories, 17(1), 139-139 (2018-09-05)
Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction
A validated antibody panel for the characterization of tau post-translational modifications.
Ebru E, et al.
Mol. Neurodegener., 12(1), 87-87 (2017)
David B Sauer et al.
eLife, 9 (2020-09-02)
Citrate, α-ketoglutarate and succinate are TCA cycle intermediates that also play essential roles in metabolic signaling and cellular regulation. These di- and tricarboxylates are imported into the cell by the divalent anion sodium symporter (DASS) family of plasma membrane transporters
Yasaman Damestani et al.
Lasers in surgery and medicine, 48(8), 782-789 (2016-10-19)
The development and feasibility of a novel nanocrystalline yttria-stabilized-zirconia (nc-YSZ) cranial implant has been recently established. The purpose of what we now call "window to the brain (WttB)" implant (or platform), is to improve patient care by providing a technique
Ibrahim Torktaz et al.
Applied microbiology and biotechnology, 102(19), 8389-8402 (2018-08-04)
The celH gene from Clostridium thermocellum encodes a protein containing 900 residues and three components, including Cel5E, Lic26a, and carbohydrate-binding domains. Cel5E is a member of the glycoside hydrolase-5 family and is a bifunctional xylanase/cellulase enzyme. We targeted a semi-hydrophobic

Verwandter Inhalt

BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

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