49641

Alkoholdehydrogenase, rekombinant aus E. coli

≥500 U/mL

• Synonym(e): Alkohol:NADP⁺-Oxidoreduktase
• CAS-Nummer: 9028-12-0
• EC-Nummer: 1.1.1.2 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00130451
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Rekombinant: expressed in E. coli
• Form: liquid
• Spezifische Aktivität: ≥500 U/mL
• Methode(n): cell based assay: suitable
• Farbe: light brownish-yellow to brown-green
• Eignung: suitable for molecular biology
• Anwendung(en): life science and biopharma
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Research area: Neuroscience

Alcohol dehydrogenase has a homodimeric structure with a co-enzyme binding domain at the C-terminal and an N-terminal catalytic domain. The active site is located at the interdomain cleft. Binding of NAD+ in the active site causes conformational changes that create the binding site for the alcohol substrate.

Anwendung

Alcohol dehydrogenase (ADH) has been used for the reversal of deficient 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay reduction in the disrupted schizophrenia 1 (DISC1-FL) and DB7 cell lysate.

Biochem./physiol. Wirkung

Alcohol dehydrogenase catalyzes the oxidative conversion of alcohol into aldehyde. The metabolism of ethanol catalyzed by alcohol dehydrogenase (ADH) results in the generation of reactive oxygen species (ROS) and nitric oxide (NO) leading to oxidative damage to mitochondria and cellular proteins and is further associated with the onset of neuroinflammation and neurological disorders.

Einheitendefinition

1 U entspricht der Enzymmenge, die 1μmol Aceton pro Minute bei pH 7.0 und 30°C (NADPH als Cofaktor) reduziert.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H319
• P-Sätze: P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2
• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves