This product is lyophilized from a solution containing phosphate buffer and sucrose and may be reconstituted in water. For testing purposes, this product is dissolved at 5 mg/mL in a cold assay buffer. See below to review the composition of the 50 mM Tris assay buffer:
1.0 ml 1 M Tris stock solution
0.4 ml of 0.5 M magnesium chloride stock solution
0.01 ml Mercapto-ethanol
Add to 15 ml H2O
adjust pH 7.3 with HCl 1 M
QS to 20 ml
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Wichtige Dokumente
48275
ββ-Galactosidase aus E. coli
suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg
Synonym(e):
β-D-Galactosid-Galactohydrolase, Lactase
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Größe auswählen
1 MG
46,30 €
5 MG
149,00 €
25 MG
409,00 €
46,30 €
Versandbereit am04. April 2025Details
Alle Fotos(1)
Größe auswählen
Ansicht ändern
1 MG
46,30 €
5 MG
149,00 €
25 MG
409,00 €
About This Item
46,30 €
Versandbereit am04. April 2025Details
Empfohlene Produkte
Form
powder
Qualität
lyophilized
Spezifische Aktivität
~140 U/mg
Mol-Gew.
Mr ~135000
Enthält
phosphate buffer as stabilizer
sucrose as stabilizer
Methode(n)
enzyme immunoassay: suitable
Versandbedingung
wet ice
Lagertemp.
−20°C
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Verwandte Kategorien
Anwendung
Anwendung: wässrige Lösung kann direkt für die Derivatisierung, ohne vorhergehende Dialyse oder Gelpermeationschromatographie eingesetzt werden
Biochem./physiol. Wirkung
β-Galactosidase spaltet Lactose in ihre Monosaccharid-Komponenten Glucose und Galactose. Außerdem katalysiert sie die Transglycosylierung von Glucose zu Allolactose, dem Induktor von β-Galactosidase, in einem Rückführungsmechanismus.
Verpackung
Bodenlose Glasflasche. Der Inhalt befindet sich innerhalb des eingeschobenen Schmelzkegels.
Physikalische Eigenschaften
Molekulargewicht des Tetramers: 465 kDa (Subunits je 116,3 kDa)
Einheitendefinition
1 U entspricht der Enzymmenge, die 1 μmol 2-Nitrophenyl-β-D-Galactopyranosid (Best. Nr. 73660) pro Minute bei einem pH-Wert von 7,8 und 37 °C oxidiert.
Sonstige Hinweise
Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590)citation; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay techniquecitation; Enzyme immunoassay of thyrptropin releasing hormone (TRH)citation; Preparation of enzyme-antibody conjugatescitation
Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Fluka®-No. 69590)[1]; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique[2]; Enzyme immunoassay of thyrptropin releasing hormone (TRH)[3]; Preparation of enzyme-antibody conjugates[4]
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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K Kato et al.
European journal of biochemistry, 62(2), 285-292 (1976-02-16)
Rabbit immunoglobulin G (IgG) was reduced by incubating with 10 mM 2-mercaptoethylamine and then treated with excess amounts of N,N'-o-phenylenedimaleimide. As a result, maleimide residues were introduced into rabbit IgG molecules. Rabbit IgG containing maleimide residues could be coupled to
Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay.
M J O'Sullivan et al.
Analytical biochemistry, 100(1), 100-108 (1979-11-15)
M Imagawa et al.
Annals of clinical biochemistry, 21 ( Pt 4), 310-317 (1984-07-01)
beta-D-galactosidase from Escherichia coli and horseradish peroxidase were evaluated as labels of Fab' in dose-response curves for human alpha-fetoprotein and human chorionic gonadotropin by sandwich enzyme immunoassay technique using fluorogenic substrates for enzyme assay. The non-specific binding of Fab'-peroxidase conjugates
M Takeyama et al.
Chemical & pharmaceutical bulletin, 40(8), 2199-2201 (1992-08-01)
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a thyrotropin releasing hormone (TRH)-like immunoreactive substance has been developed. In order to synthesize TRH-labeled beta-D-galactosidase (beta-gal), a newly devised TRH derivative, pGlu-His-Pro-NH-(CH2)6-NH2 (TRH-Hex), was employed. TRH-Hex was linked to beta-gal
Christophe Laumonnerie et al.
Development (Cambridge, England), 141(3), 594-603 (2014-01-23)
The establishment of anatomically stereotyped axonal projections is fundamental to neuronal function. While most neurons project their axons within the central nervous system (CNS), only axons of centrally born motoneurons and peripherally born sensory neurons link the CNS and peripheral
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I have already bought this one. My question is how will I reconstitute it? What diluent or buffer will I use?
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