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36798

Sigma-Aldrich

7-(Diethylamino)cumarin-3-carbohydrazid

BioReagent, suitable for fluorescence, ≥95% (HPCE)

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25 MG
333,00 €

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Versandbereit am14. April 2025Details


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25 MG
333,00 €

About This Item

Empirische Formel (Hill-System):
C14H17N3O3
CAS-Nummer:
Molekulargewicht:
275.30
Beilstein:
5574454
MDL-Nummer:
UNSPSC-Code:
12352125
PubChem Substanz-ID:
NACRES:
NA.32

333,00 €


Versandbereit am14. April 2025Details


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Produktlinie

BioReagent

Assay

≥95% (HPCE)

Löslichkeit

DMF: soluble
acetonitrile: soluble
chloroform: soluble
methanol: soluble

Fluoreszenz

λex 450 nm; λem 468 nm in methanol

Eignung

suitable for fluorescence

SMILES String

CCN(CC)c1ccc2C=C(C(=O)NN)C(=O)Oc2c1

InChI

1S/C14H17N3O3/c1-3-17(4-2)10-6-5-9-7-11(13(18)16-15)14(19)20-12(9)8-10/h5-8H,3-4,15H2,1-2H3,(H,16,18)

InChIKey

LYBMHAINSFEHRL-UHFFFAOYSA-N

Allgemeine Beschreibung

7-(Diethylamino)coumarin-3-carbohydrazide is a coumarin-hydrazide reagent typically used as a fluorescent chemical probe for labeling of cellular carbonyls.[1]

Anwendung

7-(Diethylamino)coumarin-3-carbohydrazide is used for derivatization and detection of carboxylic acid and carbonylated molecules such as lipid peroxidation products (LPP) by electrospray ionization-mass spectrometry (ESI-MS) in positive ion mode.[2] It may be used as a fluorescent label for the determination of lipid aldehydes in food products by normal phase liquid chromatography (NPLC) coupled to ESI-MS and fluorescent detector (FLD)[3] and for the derivatization of monoterpene alcohols in urine prior to analysis by high performance liquid chromatography(HPLC) with FLD coupled to ESI-MS.[4]

Verpackung

Bottomless glass bottle. Contents are inside inserted fused cone.

Sonstige Hinweise

Derivatisierungsreagens für die Bestimmung von Carbonsäuren[5]

Piktogramme

Exclamation mark

Signalwort

Warning

Gefahreneinstufungen

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Gloves


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Die Dokumentenbibliothek aufrufen

Roland J W Meesters et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 875(2), 444-450 (2008-10-11)
A method was developed for the determination of the monoterpene alcohols verbenol, myrtenol, perillyl alcohol, alpha-terpineol, Delta(3)-carene-10-ol, thymol and p-alpha,alpha-trimethylbenzylalcohol in urine samples. After an enzymatic cleavage of their glucuronide- and sulfate conjugates the monoterpene alcohols were converted in the
Ivana Milic et al.
Analytical chemistry, 85(1), 156-162 (2012-11-29)
Reactive oxygen species (ROS) and other oxidative agents such as free radicals can oxidize polyunsaturated fatty acids (PUFA) as well as PUFA in lipids. The oxidation products can undergo consecutive reactions including oxidative cleavages to yield a chemically diverse group
M.L. Grayeski et al.
Analytical Chemistry, 59, 1203-1203 (1987)
Simultaneous detection of low and high molecular weight carbonylated compounds derived from lipid peroxidation by electrospray ionization-tandem mass spectrometry
Milic I, et al.
Analytical Chemistry, 85(1), 156-162 (2012)
J C Touchstone et al.
Steroids, 56(12), 601-605 (1991-12-01)
7-Diethylaminocourmarin-3-carbohydrazide was used to label the ketone group of the tetrahydroprogesterones to form fluorescent derivatives with high sensitivity. The four isomeric 3-hydroxypregnanes separated readily on high-performance, thin-layer chromatography after derivatization. This separation was not possible with the underivatized isomers. Standards

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