This medium does not contain VEGF.
211-500
Endothelzellwachstumsmedium (500 mL)
Synonym(e):
Primary Cell Culture Media
About This Item
Empfohlene Produkte
Form
liquid
Qualitätsniveau
Haltbarkeit
6 mo.
Hersteller/Markenname
Cell Applications, Inc
Versandbedingung
wet ice
Lagertemp.
2-8°C
Allgemeine Beschreibung
Anwendung
Qualität
Angaben zur Herstellung
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Artikel
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Protokolle
Technical information for working with human pulmonary artery endothelial cells including thawing, subculturing and cryopreservation
Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.
Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
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Does the medium contain VEGF? If so, can you specify the concentration?
1 Antwort-
Hilfreich?
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If I use this medium, do I need to use tissue coated treated flasks with gelatin? Does this medium need to be supplemented with heparin or is it already included?
1 Antwort-
This medium is a complete, ready-to-use product; no supplementation is required. This formulation contains hydrocortisone, heparin and other growth factors needed to support Endothelial Cell growth. Unless a suspension culture is required, the culture vessel should be tissue culture treated. The additional use of attachment matrices such as gelatin, would be dependent on the requirements of the specific cell line or type.
Hilfreich?
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Can you please provide the full composition of the medium with the concentrations?
1 Antwort-
This item is a product of Cell Applications. The formulation is considered proprietary. However, this medium does include glucose at 1g/L, 2% Fetal Bovine Serum, as well as Penicillin, streptomycin, and Amphotericin B. Some salt components have also been published in the Safety Data Sheet.
Hilfreich?
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Do you sell glucose-free ECGM? I needed it for HUVEC cells. 0mM glucose.
1 Antwort-
This product is not a glucose-free medium. The formulation contains 5.78 mM glucose which is 5.55 mM after all the supplements are added. Unfortunately, a glucose-free ECGM medium is not currently offered.
Hilfreich?
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